.... Proepicardial cells are subsequently transferred to the myocardial surface and form the epicardium, covering the myocardium. We found that while wt1a and wt1b expression is maintained in proepicardial cells, it is downregulated in pericardial cells that contribute cardiomyocytes to the developing heart...

... ABSTRACT Endothelial cells emerge from the atrioventricular canal to form coronary blood vessels in juvenile zebrafish hearts. We find that pdgfrb is first expressed in the epicardium around the atrioventricular canal and later becomes localized mainly in the mural cells. pdgfrb mutant fish show severe...

...Yingxi Cao; Yu Xia; Joseph J. Balowski; Jianhong Ou; Lingyun Song; Alexias Safi; Timothy Curtis; Gregory E. Crawford; Kenneth D. Poss; Jingli Cao ABSTRACT The epicardium is a mesothelial tissue layer that envelops the heart. Cardiac injury activates dynamic gene expression programs in epicardial...

... development of the epicardium, accompanied by downregulation of Wilms’ tumor 1. Agrin enhanced EMT in human embryonic stem cell-derived epicardial-like cells by decreasing β-catenin and promoting pFAK localization at focal adhesions, and promoted the aggregation of dystroglycan within the Golgi apparatus...

...Laure Gambardella; Sophie A. McManus; Victoria Moignard; Derya Sebukhan; Agathe Delaune; Simon Andrews; William G. Bernard; Maura A. Morrison; Paul R. Riley; Berthold Göttgens; Nicolas Gambardella Le Novère; Sanjay Sinha ABSTRACT The murine developing epicardium heterogeneously expresses...

...Víctor Velecela; Alejo Torres-Cano; Ana García-Melero; Marina Ramiro-Pareta; Claudia Müller-Sánchez; Marc Segarra-Mondejar; You-Ying Chau; Begoña Campos-Bonilla; Manuel Reina; Francesc X. Soriano; Nicholas D. Hastie; Fernando O. Martínez; Ofelia M. Martínez-Estrada ABSTRACT The epicardium plays...

...Vanessa Lowe; Laura Wisniewski; Jacob Sayers; Ian Evans; Paul Frankel; Nadia Mercader-Huber; Ian C. Zachary; Caroline Pellet-Many ABSTRACT Unlike adult mammals, zebrafish can regenerate their heart. A key mechanism for regeneration is the activation of the epicardium, leading to the establishment...

...Filipa C. Simões; Paul R. Riley The epicardium plays a key role during cardiac development, homeostasis and repair, and has thus emerged as a potential target in the treatment of cardiovascular disease. However, therapeutically manipulating the epicardium and epicardium-derived cells (EPDCs...

... the molecular mechanisms that regulate coronary vessel formation is crucial for preventing and treating the disease. We report a zebrafish gene-trap vinculin b ( vclb ) mutant that displays abnormal coronary vessel development among multiple cardiac defects. The mutant shows overproliferation of epicardium...

...Panna Tandon; Caralynn M. Wilczewski; Clara E. Williams; Frank L. Conlon The development of the vertebrate embryonic heart occurs by hyperplastic growth as well as the incorporation of cells from tissues outside of the initial heart field. Amongst these tissues is the epicardium, a cell structure...

... covering the heart is activated by injury and aids muscle regeneration through paracrine effects and as a multipotent cell source, and has received recent attention as a target in cardiac repair strategies. Although it is recognized that epicardium is required for muscle regeneration and itself has high...

...Catherine A. Risebro; Joaquim Miguel Vieira; Linda Klotz; Paul R. Riley The epicardium is essential for mammalian heart development. At present, our understanding of the timing and morphogenetic events leading to the formation of the human epicardium has essentially been extrapolated from model...

...Dharini Iyer; Laure Gambardella; William G. Bernard; Felipe Serrano; Victoria L. Mascetti; Roger A. Pedersen; Amarnath Talasila; Sanjay Sinha The epicardium has emerged as a multipotent cardiovascular progenitor source with therapeutic potential for coronary smooth muscle cell, cardiac fibroblast...

...Michael A. Trembley; Lissette S. Velasquez; Karen L. de Mesy Bentley; Eric M. Small An important pool of cardiovascular progenitor cells arises from the epicardium, a single layer of mesothelium lining the heart. Epicardium-derived progenitor cell (EPDC) formation requires epithelial-to-mesenchymal...

... of Biologists Ltd 2013 Heart regeneration Chemical screen Cardiomyocyte Cell proliferation Epicardium Endocardium Zebrafish A fundamental cellular block to heart regeneration in adult mammals is the low capacity of mature mammalian cardiomyocytes to undergo proliferation after injury...

..., commitment and differentiation in multiple cell lineages during development. Here, we describe the role of a bHLH transcription factor, Tcf21 (epicardin/Pod1/capsulin), in specification of the cardiac fibroblast lineage. In the developing heart, the epicardium constitutes the primary source of progenitor...

...Seung Tae Baek; Michelle D. Tallquist The epicardium is the primary source of coronary vascular smooth muscle cells (cVSMCs) and fibroblasts that reside in the compact myocardium. To form these epicardial-derived cells (EPDCs), the epicardium undergoes the process of epithelial to mesenchymal...

... interests statement The authors declare no competing financial interests. 9 6 2011 © 2011. Cardiomyocyte Genetic ablation Heart regeneration Zebrafish Epicardium Endocardium Heart failure To test for recovery, we performed the same assays in Z-CAT animals...

...Mónica Vega-Hernández; Attila Kovacs; Stijn De Langhe; David M. Ornitz The epicardium serves as a source of growth factors that regulate myocardial proliferation and as a source of epicardial-derived cells (EPDC), which give rise to interstitial cardiac fibroblasts and perivascular cells...

...Kazu Kikuchi; Vikas Gupta; Jinhu Wang; Jennifer E. Holdway; Airon A. Wills; Yi Fang; Kenneth D. Poss Recent lineage-tracing studies have produced conflicting results about whether the epicardium is a source of cardiac muscle cells during heart development. Here, we examined the developmental...