... during retinal angiogenesis. Macrophage Pericytes Vascular smooth muscle cells Endothelial cells Angiogenesis Japan Society for the Promotion of Science http://dx.doi.org/10.13039/501100001691 20K07248 22K15359 Angiogenesis involves the formation of new blood vessels from...

... arteriovenous malformations. Single-cell RNA-sequencing analysis of akt Δ/Δ endothelial cells corroborates the impairment of arterial, yet not venous, cell specification. Notably, endothelial specific expression of ligand-independent activation of Notch or constitutively active Akt1 were sufficient to re...

... (TEM) 5 mediates endothelial cell survival during angiogenesis by linking integrin α(v)β3 to glycosaminoglycans . J. Biol. Chem. 281 , 34179 - 34188 . 10.1074/jbc.M605291200 Vallon , M. , Yuki , K. , Nguyen , T. D. , Chang , J. , Yuan , J. , Siepe , D. , Miao...

... that inhibitory SMAD6 functions in endothelial cells to negatively regulate ALK1-mediated responses, and it is required to prevent vessel dysmorphogenesis and hemorrhage in the embryonic liver vasculature. Reduced Alk1 gene dosage rescued embryonic hepatic hemorrhage and microvascular capillarization induced...

... mechanisms remain unknown. In this study, we show that FGR endothelial cells demonstrate inherently reduced migratory capacity despite the presence of fibronectin, a matrix protein abundant in placental stroma that displays abnormal organization in FGR placentas. Thus, we hypothesized that aberrant...

... tissue interactions at key time points in the mouse embryo. We demonstrate the utility of the approach by providing volumetric data, 3D distribution of three main cellular components (epithelial, mesenchymal and endothelial cells) within the developing pancreas, and quantification of their relative...

... and Rasip1. Yellow arrowheads indicate absence of Rasip1 from endothelial cell junctions. (E) Quantification of the relative intensity of Rasip1 localized at junctions compared with that on the apical membrane. Ratio=junctional Rasip1/apical Rasip1 referenced by VE-Cadherin (junction). Control-MO injected...

... system. These phenotypes arise from abnormal gene expression patterns that reflect defective kinase signaling, including TGFβ and several receptor tyrosine kinase pathways. USP22 deletion in endothelial cells and pericytes that are induced from embryonic stem cells also hinders these signaling cascades...

... stress, human coronary artery endothelial cells failed to align when either BMPs were inhibited or SMAD4 was depleted. In contrast to control cells, SMAD4- deficient cells did not migrate against the direction of shear stress and increased proliferation rates specifically under flow. Similar alterations...

...Jessica E. Nesmith; John C. Chappell; Julia G. Cluceru; Victoria L. Bautch Blood vessel formation is essential for vertebrate development and is primarily achieved by angiogenesis – endothelial cell sprouting from pre-existing vessels. Vessel networks expand when sprouts form new connections...

... explants, we determined that VEGFR2-dependent signaling is required for salivary gland epithelial patterning. To test directly for a requirement for endothelial cells in instructive epithelial patterning, we developed a novel ex vivo cell fractionation/reconstitution assay. Immuno-depletion of CD31...

...Charles Betz; Anna Lenard; Heinz-Georg Belting; Markus Affolter Vascular networks are formed and maintained through a multitude of angiogenic processes, such as sprouting, anastomosis and pruning. Only recently has it become possible to study the behavior of the endothelial cells that contribute...

... interests statement The authors declare no competing financial interests. 10 5 2013 © 2013. Published by The Company of Biologists Ltd 2013 Vascular development Angiogenesis Endothelial cells Notch Mouse The vertebrate body contains an extensive tubular system...

... perfusion is created through not only the generation of new blood vessels but also the remodeling and maintenance of endothelial cells via apoptotic and cell survival pathways. Here, we show that UBIAD1, a vitamin K2/menaquinone-4 biosynthetic enzyme, functions cell-autonomously to regulate endothelial cell...

...Daniela Malan; Andrea Elischer; Michael Hesse; Sara A. Wickström; Bernd K. Fleischmann; Wilhelm Bloch Integrin linked kinase (ILK) connects the ILK-Pinch-Parvin complex with integrin adhesion sites. Because of the functional relevance of integrin-linked signaling for endothelial cell (EC) biology...

...Susumu Sakimoto; Hiroyasu Kidoya; Hisamichi Naito; Motohiro Kamei; Hirokazu Sakaguchi; Nobuhito Goda; Akiyoshi Fukamizu; Kohji Nishida; Nobuyuki Takakura Interactions between astrocytes and endothelial cells (ECs) are crucial for retinal vascular formation. Astrocytes induce migration...

... interests statement The authors declare no competing financial interests. 4 12 2011 © 2012. Hox genes Endothelial cells Enhancer Placenta Transcriptional regulation Vascular progenitors Mouse Chick The Hox gene family is well known for its major role...

... revealed that endothelial progenitors, like second/anterior heart field progenitors, migrate to, and enter, the heart from the arterial pole. Furthermore, embryonic endothelial cells implanted into the cardiac crescent contribute to the endocardium, but not to the myocardium. In mouse, lineage analysis...

.... Using clonal and explant culture experiments, we show that these cells are a stem cell population that can differentiate into myocardium, smooth muscle and endothelial cells. The multipotent stem cells proliferate or differentiate into different cardiovascular cell fates through activation or inhibition...

...Gavin Thurston; Quan Wang; Fabienne Baffert; John Rudge; Nicholas Papadopoulos; Danielle Jean-Guillaume; Stanley Wiegand; George D. Yancopoulos; Donald M. McDonald Early in development, endothelial cells proliferate, coalesce, and sprout to form a primitive plexus of undifferentiated microvessels...