... the mouse segmentation clock. Ministry of Education, Culture, Sports, Science, and Technology 16H06480 Core Research for Evolutional Science and Technology 10.13039/501100003382 JPMJCR12W2 Chemical library screening Embryonic stem cell Induced presomitic mesoderm...

... no competing or financial interests. © 2017. Published by The Company of Biologists Ltd 2017 Summary: This Meeting Review summarises the major themes and results presented at the second 'From Stem Cells to Human Development' meeting, held in October 2016. Development Embryonic stem cell...

... characterized by loss of heterochromatin, activation of tandem repeats, oxidative stress and Rip3-associated necroptosis. In the human retina, BMI1 was preferentially expressed in cones at heterochromatic foci. BMI1 inactivation in human embryonic stem cells was compatible with retinal induction but impaired...

... a minority of the embryonic stem cell (ESC) inoculum contributes to the adult chimaera. How incoming cells are chosen for integration or elimination remains unclear. By comparing a heterogeneous mix of undifferentiated and differentiating ESCs (serum/LIF) with more homogeneous undifferentiated culture (2i...

...Sophie M. Morgani; Joshua M. Brickman Embryonic stem cells (ESCs) are pluripotent cell lines that can be maintained indefinitely in an early developmental state. ESC culture conditions almost always require the cytokine LIF to maintain self-renewal. As ESCs are not homogeneous but contain multiple...

...Meritxell Huch; Bon-Kyoung Koo In vitro three-dimensional (3D) cultures are emerging as novel systems with which to study tissue development, organogenesis and stem cell behavior ex vivo . When grown in a 3D environment, embryonic stem cells (ESCs) self-organize into organoids and acquire the right...

...Guang Li; Karolina Plonowska; Rajarajan Kuppusamy; Anthony Sturzu; Sean M. Wu The transcriptional profiles of cardiac cells derived from murine embryos and from mouse embryonic stem cells (mESCs) have primarily been studied within a cell population. However, the characterization of gene expression...

...Satoshi Ohtsuka; Hitoshi Niwa The requirement of leukemia inhibitory factor (LIF) for the establishment and maintenance of mouse embryonic stem cells (ESCs) depends on the genetic background of the ESC origin. To reveal the molecular basis of the strain-dependent function of LIF, we compared...

..., as they all display a loss of posterior somites and PSM ( Fujimoto and Yanagisawa, 1979 ; Chapman and Papaioannou, 1998 ; Yoon et al., 2000 ; Yoon and Wold, 2000 ). However, gain-of-function studies on Tbx6 in mice ( Wehn and Chapman, 2010 ) and T in embryonic stem cells (ESCs) ( Lindsley et al., 2008 ) do...

... is known about how this crucial subtype of motor neuron is generated during embryogenesis. Here, we used direct differentiation of motor neurons from mouse embryonic stem cells as a tool to identify genes that direct phrenic neuron identity. We find that three determinants, Pou3f1, Hoxa5 and Notch, act...

... there is access to appropriate tissue and sufficient numbers of articular chondrocytes. Embryonic stem cells (ESCs) represent a potentially unlimited source of chondrocytes and tissues as they can generate a broad spectrum of cell types under appropriate conditions in vitro . Here, we demonstrate that mouse ESC...

... intensities of G1 (red fluorescence) and S/G2-M (green) that are associated with self-renewal and differentiation of single murine neural stem/progenitor cells (NSCs) and embryonic stem cells (ESCs). We further exploited these distinctive features using fluorescence-activated cell sorting to select...

... and contribute to pathogenesis of atherosclerosis and the formation of arterial aneurysms ( Raines, 2000 ). Angiogenesis Embryonic stem cell Myocardin-related transcription factor B/MKL2 Mouse Transforming growth factor β References Astrof S. , Hynes R. O. ( 2009...

...-Porath I. , Thomson M. W. , Carey V. J. , Ge R. , Bell G. W. , Regev A. , Weinberg R. A. ( 2008 ). An embryonic stem cell-like gene expression signature in poorly differentiated aggressive human tumors . Nat. Genet. 40 , 499 – 507 . Brannon M...

... for correspondence (e-mail: [email protected] ) 15 2 2008 © 2008. 2008 Bmp4 Embryonic stem cell Hemangioblast Hematopoiesis VEGF Mouse Upon differentiation, mouse embryonic stem (ES) cells can give rise to primitive and definitive hematopoietic precursors, an in vitro process...

... into the uterine endometrium. However, Tead4 -/- embryos can produce embryonic stem cells, a derivative of ICM, and if the Tead4 allele is not disrupted until after implantation, then Tead4 -/- embryos complete development. Thus, Tead4 is the earliest gene shown to be uniquely required for specification...

... pluripotency is derived from an ancestral function of this class of proteins to maintain multipotency. * Author for correspondence (e-mail: [email protected] ) 3 3 2006 © 2006. 2006 Gastrulation Self-renewal Embryonic stem cell Lineage commitment Pou protein Pou5f1...

...Ariel J. Levine; Ali H. Brivanlou The TGFβ superfamily of ligands plays key functions in development and disease. In both human and mouse embryonic stem cells, a member of this family, GDF3, is specifically expressed in the pluripotent state. We show that GDF3 is an inhibitor of its own subfamily...

...Kyunghee Choi; Marion Kennedy; Alexander Kazarov; John C. Papadimitriou; Gordon Keller ABSTRACT Embryonic stem cell-derived embryoid bodies contain a unique precursor population which, in response to vascular endothelial growth factor, gives rise to blast colonies in semi-solid medium. Upon...

...Joy T. Yang; Helen Rayburn; Richard O. Hynes ABSTRACT A loss of function mutation of the murineα 5 integrin gene generated by gene targeting in embryonic stem cells is a recessive embryonic lethal. The mutant embryos start to show observable defects by day 9 of gestation and die around day 10-11...