... plate, consistent with a cell-autonomous disturbance of convergent extension. Inhibitor studies in whole-embryo culture demonstrated a requirement for signalling via RhoA-Rho kinase, but not jun N-terminal kinase, in convergent extension and the onset of neural tube closure. These findings identify...

... the neural tube to the developing heart. A cardiac neural crest lineage is also known to exist in mammals, although detailed information on its axial level of origin and migratory pattern are lacking. We used focal cell labelling and orthotopic grafting, followed by whole embryo culture, to determine...

... Anterior visceral endoderm Patterning Morphogenesis Migration Embryo culture Time lapse imaging The early post-implantation mouse embryo consists of three tissues arranged like a cylinder, with extra-embryonic ectoderm positioned proximally, epiblast distally and visceral endoderm surrounding...

.... 2002 Neurulation Neural plate Morphogenesis Embryo culture Notochord patched Neurulation is the embryonic process in which the neural plate, a specialised region of ectoderm on the dorsal surface of the embryo, undergoes shaping and folding to form the neural tube. Failure of neural...

... phenotype is strikingly different from that of embryos lacking RARα and RARβ (Aα/Aβmutants), in which we have previously shown that the territory corresponding to R5 and R6 is markedly enlarged. Administration of a pan-RAR antagonist at E8.0 to wild-type embryos cultured in vitro results in an Aα/Aβ-like...

... crest Hox gene Pax gene Fgf Embryo culture Mouse Pharyngeal arches (PA) are transient bulges of the embryonic surface that develop in a cranial to caudal sequence between the head and the heart and are separated by evaginations of the pharyngeal endoderm, the pharyngeal pouches. Adult...

... Bone morphogenetic protein 2 (BMP2) Cardiac progenitor cell Embryo culture In situ hybridization Intercalated disc Cadherin signaling Cardiac progenitor cells originate from the rostral half of the primitive streak and form a pair of heart-forming fields within lateral plate mesoderm...

... with continued normal fetal development in vivo. * Author for correspondence 26 01 1995 © 1995 by Company of Biologists 1995 macrophage colony-stimulating factor CSF-1 blastocyst trophoblast cell preimplantation development embryo culture Several receptors that recognize...

... sufficiently similar to justify the use of cultured embryos in subsequent experiments. * F.A.B. and A.S.W S. contributed equally to this study 26 06 1993 © 1991 by Company of Biologists 1991 neural tube morphogenesis mouse mutant embryo culture curvature curly tail ( ct...

... Leukemia Virus mouse embryo Lac Z expressing vector long terminal repeat embryo culture © 1990 by Company of Biologists 1990 ‡ Present address: Immunologic Pharmaceutical, 1 Kendall Square, Building 600, Cambridge, MA 02139, USA † To whom reprint requests should be addressed...

... microinjected orthotopically into host embryos of the same developmental stage except for area 1 (forebrain) grafts which were, for technical reasons, injected into area 2. After various periods of time in whole embryo culture, the embryos were examined by immunohistochemical staining in order to determine...

.... B. ( 1981 ). The role of the visceral yolk sac in mediating protein utilization by rat embryos cultured in vitro . J. Embryol. exp. Morph . 66 , 223 – 234 . Freeman , S. J. , Brent , R. L. & Lloyd , J. B. ( 1982 ). The effect of teratogenic antiserum in yolk sac...