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Keywords: Electroporation
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Journal Articles
Journal: Development
Development (2014) 141 (18): 3605–3611.
Published: 15 September 2014
.... Competing interests The authors declare no competing financial interests. 19 6 2014 30 7 2014 © 2014. Published by The Company of Biologists Ltd 2014 To easily count the number of nuclei of electroporated cells, we constructed a plasmid (Tol2-CAGGS-NLSmCherry-IRES-mEGFP...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2014) 141 (12): 2494–2505.
Published: 15 June 2014
... interference (RNAi), was performed in pre-migratory FNC cells. Soon after electroporation of RNAi molecules, Smad1 inactivation abolished the expression of Foxg1 in the chick telencephalon, resulting in dramatic microcephaly and partial holoprosencephaly. In addition, the depletion of Foxg1 activity altered...
Journal Articles
Journal: Development
Development (2014) 141 (7): 1589–1598.
Published: 1 April 2014
... and therefore incapable of genomic transposition (representing genomic and episomal contributions, respectively). As soon as 2 days after electroporation and continued at 7 and 14 days after transfection ( supplementary material Fig. S3 ), cells expressing mCherry were found across different transfected areas...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2012) 139 (22): 4261–4270.
Published: 15 November 2012
... boundaries with the adjacent neuroepithelium. Through a combination of in ovo transplantation, co-culture and electroporation techniques in chick embryos between embryonic days 3 and 6, we demonstrate that organiser properties are maintained by interactions between the non-neural roof plate and the neural...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2009) 136 (15): 2579–2589.
Published: 1 August 2009
.... * Author for correspondence (e-mail: takashi.mikawa@ucsf.edu ) 18 5 2009 © 2009. 2009 Eye Retina Pigmented epithelium Electroporation Sox genes Chick embryo The retina originates from a bilateral evagination of the forebrain, called the optic vesicle (OV). As the overlying...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2007) 134 (21): 3805–3814.
Published: 1 November 2007
... resolves to otic, lens and olfactory placodes. Ectopic expression of Spalt4 by electroporation is sufficient to induce invagination of non-placodal head ectoderm and prevent neurogenic placodes from contributing to cranial ganglia. Conversely, loss of S palt4 function in the otic placode results...
Journal Articles
Journal: Development
Development (2006) 133 (10): 1923–1931.
Published: 15 May 2006
... hybridization analysis shows that at least four classic cadherins, cadherin 6 (Cad6), cadherin 8 (Cad8), cadherin11 (Cad11) and N-cadherin (Ncad), are expressed in the migratory streams of lateral reticular nucleus and external cuneate nucleus (LRN/ECN) neurons. Functional analysis performed by electroporation...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2005) 132 (6): 1295–1304.
Published: 15 March 2005
... occupy progressively upper layers. The underlying molecular mechanisms of neurogenesis, however, are relatively unknown. In this study, we devised a system where the Notch pathway was activated spatiotemporally in the cortex by in vivo electroporation and Cre -mediated DNA recombination. Electroporation...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2005) 132 (3): 541–551.
Published: 1 February 2005
... as hair cells so as to generate the fine-grained mixture of hair cells and supporting cells. * Author for correspondence (e-mail: julian.lewis@cancer.org.uk ) 9 11 2004 ©2005. 2005 Notch Inner ear Chick Hair cells Electroporation Lateral inhibition Prosensory determination...
Journal Articles
Journal: Development
Development (2004) 131 (4): 713–723.
Published: 15 February 2004
... to investigate the effect of electroporation of the mouse Myf5 and MyoD genes in the embryonic neural tube. We found that misexpression of either mouse Myf5 or MyoD in the chick neural tube leads to ectopic skeletal muscle differentiation, assayed by the expression of the myosin heavy chains in the neural tube...
Journal Articles
Journal: Development
Development (2004) 131 (4): 787–796.
Published: 15 February 2004
...., Nomura, T. and Osumi, N. ( 2002 ). Manipulating gene expressions by electroporation in the developing brain of mammalian embryos. Differentiation 70 , 155 -162. Toresson, H., Potter, S. S. and Campbell, K. ( 2000 ). Genetic control of dorsal-ventral identity in the telencephalon: opposing roles...
Journal Articles
Journal: Development
Development (2003) 130 (14): 3269–3281.
Published: 15 July 2003
... gradients are involved in establishing borders of gene expression domains in different regions of the nervous system. In ovo electroporation of chick embryos was performed as described previously ( Funahashi et al.,1999 ) using a BTX electroporator (Electro Square Porator, ECM 830), with the following...
Journal Articles
Journal: Development
Development (2003) 130 (11): 2407–2418.
Published: 1 June 2003
... repellents Chick visual system Electroporation Optic tectum Eph receptors Gradients Recombinant retrovirus Topographic maps Topographic maps are a fundamental organizational feature of most axonal connections in the brain. The dominant model system for studying map development...
Journal Articles
Journal: Development
Development (2003) 130 (3): 587–598.
Published: 1 February 2003
... is where Wnt signaling is supposedly activated, expressed a set of molecular markers that are characteristic of the progenitor cells in the ciliary marginal zone. Overexpression of Wnt2b by use of in ovo electroporation in the central retina inhibited neuronal differentiation and induced the progenitor...
Journal Articles
Journal: Development
Development (2002) 129 (22): 5103–5115.
Published: 15 November 2002
... treatments at these stages. We demonstrated by in ovo electroporation of a dominant negative retinoid receptor construct( dnRAR ) that retinoid signaling is required to initiate expression. Elevating CDX activity by ectopic expression of an activated form of a Xenopus Cdx gene ( XcadVP16 ) in the hindbrain...
Journal Articles
Journal: Development
Development (2002) 129 (18): 4301–4313.
Published: 15 September 2002
... crest Facial skeleton Hox Electroporation Chimera Chick Quail Cell lineage studies carried out in the avian embryo have established the triple origin of the cells from which the head skeleton is built ( Couly et al., 1993 ; Le Douarin and Kalcheim, 1999 ). The posterior part of the skull...
Journal Articles
Journal: Development
Development (2002) 129 (1): 245–252.
Published: 1 January 2002
... of the phenotype of GABAergic neurons, we adapted the electroporation method to ectopically express DLX proteins in slice cultures of the mouse embryonic cerebral cortex. This approach showed that ectopic expression of Dlx2 and Dlx5 induced the expression of glutamic acid decarboxylases (GADs), the enzymes...