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Keywords: Electroporation
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Journal Articles
Journal: Development
Development (2014) 141 (18): 3605–3611.
Published: 15 September 2014
... is unknown. We electroporated the medial portion of newly formed epithelial somites in the interlimb region as previously described ( Scaal et al., 2004 ). This leads to the specific labeling of the medial border of the dermomyotome (the DML; Fig. 1 A), from which emerge the first myocytes...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2014) 141 (12): 2494–2505.
Published: 15 June 2014
... interference (RNAi), was performed in pre-migratory FNC cells. Soon after electroporation of RNAi molecules, Smad1 inactivation abolished the expression of Foxg1 in the chick telencephalon, resulting in dramatic microcephaly and partial holoprosencephaly. In addition, the depletion of Foxg1 activity altered...
Journal Articles
Journal: Development
Development (2014) 141 (7): 1589–1598.
Published: 1 April 2014
... Electroporation Cerebral cortex Wulst Dorsal ventricular ridge The goal of cell lineage analysis is to provide information on the number, distribution, phenotype and functional integration of all cells derived from the same single embryonic stem cell or progenitor population ( Buckingham...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2012) 139 (22): 4261–4270.
Published: 15 November 2012
... boundaries with the adjacent neuroepithelium. Through a combination of in ovo transplantation, co-culture and electroporation techniques in chick embryos between embryonic days 3 and 6, we demonstrate that organiser properties are maintained by interactions between the non-neural roof plate and the neural...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2009) 136 (15): 2579–2589.
Published: 1 August 2009
.... * Author for correspondence (e-mail: takashi.mikawa@ucsf.edu ) 18 5 2009 © 2009. 2009 Eye Retina Pigmented epithelium Electroporation Sox genes Chick embryo The retina originates from a bilateral evagination of the forebrain, called the optic vesicle (OV). As the overlying...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2007) 134 (21): 3805–3814.
Published: 1 November 2007
... resolves to otic, lens and olfactory placodes. Ectopic expression of Spalt4 by electroporation is sufficient to induce invagination of non-placodal head ectoderm and prevent neurogenic placodes from contributing to cranial ganglia. Conversely, loss of S palt4 function in the otic placode results...
Journal Articles
Journal: Development
Development (2006) 133 (10): 1923–1931.
Published: 15 May 2006
... hybridization analysis shows that at least four classic cadherins, cadherin 6 (Cad6), cadherin 8 (Cad8), cadherin11 (Cad11) and N-cadherin (Ncad), are expressed in the migratory streams of lateral reticular nucleus and external cuneate nucleus (LRN/ECN) neurons. Functional analysis performed by electroporation...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2005) 132 (6): 1295–1304.
Published: 15 March 2005
... occupy progressively upper layers. The underlying molecular mechanisms of neurogenesis, however, are relatively unknown. In this study, we devised a system where the Notch pathway was activated spatiotemporally in the cortex by in vivo electroporation and Cre -mediated DNA recombination. Electroporation...
Includes: Supplementary data
Journal Articles
Journal Articles
Journal: Development
Development (2004) 131 (4): 713–723.
Published: 15 February 2004
... to investigate the effect of electroporation of the mouse Myf5 and MyoD genes in the embryonic neural tube. We found that misexpression of either mouse Myf5 or MyoD in the chick neural tube leads to ectopic skeletal muscle differentiation, assayed by the expression of the myosin heavy chains in the neural tube...
Journal Articles
Journal: Development
Development (2004) 131 (4): 787–796.
Published: 15 February 2004
... of the telencephalon. * Author for correspondence (e-mail: osumi@mail.cc.tohoku.ac.jp ) 5 11 2003 © 2004. 2004 Pax6 Olfactory bulb Cell migration Telencephalon Whole embryo culture Brain organ culture Electroporation Rats The olfactory bulbs (OBs) are structures...
Journal Articles
Journal: Development
Development (2003) 130 (14): 3269–3281.
Published: 15 July 2003
... Electroporation The generation of a large variety of neuronal and glial cell types at defined positions is essential for the development of a functional nervous system. The establishment of neuronal and glial diversity is initiated by patterning of the neural tube along the anteroposterior...
Journal Articles
Journal Articles
Journal: Development
Development (2003) 130 (3): 587–598.
Published: 1 February 2003
... is where Wnt signaling is supposedly activated, expressed a set of molecular markers that are characteristic of the progenitor cells in the ciliary marginal zone. Overexpression of Wnt2b by use of in ovo electroporation in the central retina inhibited neuronal differentiation and induced the progenitor...
Journal Articles
Journal Articles
Journal: Development
Development (2002) 129 (18): 4301–4313.
Published: 15 September 2002
... as described by Etchevers et al. ( Etchevers et al., 2001 ). Ectopic Hoxa2 gene expression was targeted to the anterior neural fold of 5-6 ss chick embryos by electroporation of a Hoxa2 retroviral construct containing Hoxa2 cDNA that is active in chick and not in quail cells ( Grammatopoulos et al...
Journal Articles
Journal: Development
Development (2002) 129 (1): 245–252.
Published: 1 January 2002
... of the phenotype of GABAergic neurons, we adapted the electroporation method to ectopically express DLX proteins in slice cultures of the mouse embryonic cerebral cortex. This approach showed that ectopic expression of Dlx2 and Dlx5 induced the expression of glutamic acid decarboxylases (GADs), the enzymes...
Journal Articles
Journal: Development
Development (2001) 128 (4): 561–569.
Published: 15 February 2001
... with exogenous cadherin-6 were generated to straddle the cortico-straital boundary by electroporation at embryonic day 11.0, ectopic cadherin-6-expressing cortical cells were sorted into the striatal compartment, and the reverse was the trend for ectopic R-cadherin-expressing striatal cells. Although cadherin-6...