...Yinan Zhao; Dandan Bai; You Wu; Dan Zhang; Mengying Liu; Yingpu Tian; Jinhua Lu; Haibin Wang; Shaorong Gao; Zhongxian Lu ABSTRACT How maternal Ezh1 and Ezh2 function in H3K27 methylation in vivo in pre-implantation embryos and during embryonic development is not clear. Here, we have deleted Ezh1...

... with repressed genes, but the modification is not sufficient to induce repression and, in some instances, is not required. We blocked full methylation of H3K27 with both a small molecule inhibitor, GSK343, and by introducing a point mutation into EZH2, the catalytic subunit of PRC2, in the mouse CJ7 cell line...

... during embryonic development. Among them, Ezh2 is responsible for catalyzing the epigenetic repressive mark H3K27me3 and is essential for animal development. The ability of zebrafish embryos lacking both maternal and zygotic ezh2 to form a normal body plan provides a unique model for comprehensively...

... (Krt5 + ) basal cells and ectopic expression of squamous-like differentiation markers. Deletion of Ezh2 , the major enzymatic component of PRC2, causes upregulation of Upk3a + superficial cells. Unexpectedly, Eed and Eed / Ezh2 double mutants exhibit delayed superficial cell differentiation. Furthermore...

... such as idiopathic pulmonary fibrosis and chronic obstructive pulmonary disease, although the factors that regulate early lung mesoderm development are unknown. We show in mouse that the PRC2 component Ezh2 is required to restrict smooth muscle differentiation in the developing lung mesothelium. Mesodermal loss...

..., we report that cerebellar deletion of Ezh2, the methyltransferase subunit of the PRC2 complex, results in reduced H3K27me3 and profound transcriptional dysregulation, including that of a set of transcription factors directly involved in cerebellar neuronal cell-type specification and differentiation...

.... Here, we explored the role of the histone methyltransferase Ezh2 during lung lineage determination. Loss of Ezh2 in the lung epithelium leads to defective lung formation and perinatal mortality. We show that Ezh2 is crucial for airway lineage specification and alveolarization. Using optical projection...

... to modulate neural crest development in Xenopus . The PRC2 core components Eed, Ezh2 and Suz12 are expressed in the neural crest cells and are required for neural crest marker expression. Knockdown of Ezh2, the catalytic subunit of PRC2 for histone H3K27 methylation, results in defects in neural crest...

... blood vessels are poorly understood. Here, we show that the histone methyltransferase Ezh2 maintains integrity of the developing vasculature by repressing a transcriptional program that activates expression of Mmp9 . Inactivation of Ezh2 in developing mouse endothelium caused embryonic lethality...

... pattern of osteochondrogenic competence is achieved remains to be elucidated. Here we demonstrate that Hox gene expression and osteochondrogenesis are controlled by epigenetic mechanisms. Ezh2, which is a component of polycomb repressive complex 2 (PRC2), catalyzes trimethylation of lysine 27 in histone 3...

... , PRC2 regulates the progression of retinal progenitors from proliferation to differentiation. We show that the PRC2 core components are enriched in retinal progenitors and downregulated in differentiated cells. Knockdown of the PRC2 core component Ezh2 leads to reduced retinal progenitor proliferation...

... formation. Using conditional mutagenesis at different time points, we show that the histone methyltransferase Ezh2 is an upstream regulator of anteroposterior prepattern at an early stage. Mutants exhibit posteriorised limb bud identity. During later limb bud stages, Ezh2 is essential for cell survival...

... ( [email protected] ) Competing interests statement 21 6 2010 © 2010. Neural development BMP pathway Epigenetic regulation Histone methylation EZH2 JMJD3 Chick neural tube During embryogenesis, multipotent neuroepithelial precursor cells originate...

...Sylvia Erhardt; I-hsin Su; Robert Schneider; Sheila Barton; Andrew J. Bannister; Laura Perez-Burgos; Thomas Jenuwein; Tony Kouzarides; Alexander Tarakhovsky; M. Azim Surani Enhancer of zeste 2 (Ezh2), a SET domain-containing protein, is crucial for development in many model organisms, including...