...Tüzer Kalkan; Nelly Olova; Mila Roode; Carla Mulas; Heather J. Lee; Isabelle Nett; Hendrik Marks; Rachael Walker; Hendrik G. Stunnenberg; Kathryn S. Lilley; Jennifer Nichols; Wolf Reik; Paul Bertone; Austin Smith Mouse embryonic stem (ES) cells are locked into self-renewal by shielding from...

... inhibit neural differentiation in ESCs by acting at enhancer sequences independently of the NuRD complex, and are dispensable for the maintenance of pluripotency. Sall4 NuRD ES cells Enhancer Transcription factor Co-repressor Embryonic stem cells (ESCs) have the potential to form any...

... 11 2013 24 3 2014 © 2014. Published by The Company of Biologists Ltd 2014 Ssm1 KRAB-zinc finger genes DNA methylation Gene inactivation ES cells Mouse development The role of epigenetic modification in the control of gene expression has been abundantly demonstrated...

....2013.12.008 . Li M. A. , Pettitt S. J. , Yusa K. , Bradley A. ( 2010 ). Genome-wide forward genetic screens in mouse ES cells . Methods Enzymol. 477 , 217 – 242 . Li W. , Shuai L. , Wan H. , Dong M. , Wang M. , Sang L. , Feng C. , Luo...

... interests. 7 5 2013 © 2013. Published by The Company of Biologists Ltd 2013 Long non-coding RNAs Ribosome profiling Embryogenesis Zebrafish ES cells Long non-coding RNAs (lncRNAs) have emerged as important regulators of gene expression during development ( Pauli et...

... distinct multimeric protein complexes, the Polycomb repressive complexes 1 (PRC1) and 2(PRC2). Although PRC2 has been shown to share target genes with the core transcription network, including Oct3/4, to maintain embryonic stem (ES)cells, it is still unclear whether PcG proteins and the core transcription...

... of the hemangioblast, an embryonic progenitor of the hematopoietic and endothelial lineages. Eng -/- , Eng +/- and Eng +/+ mouse embryonic stem (ES) cells were differentiated as embryoid bodies (EBs) and assayed for blast colony-forming cells (BL-CFCs). Our results showed a profound reduction in hemangioblast...

...Keisuke Kaji; Jennifer Nichols; Brian Hendrich Mbd3 is a core component of the NuRD (Nucleosome Remodeling and Histone Deacetylation) co-repressor complex, and NuRD-mediated silencing has been implicated in cell fate decisions in a number of contexts. Mbd3-deficient embryonic stem (ES) cells made...

...Peter Cartwright; Cameron McLean; Allan Sheppard; Duane Rivett; Karen Jones; Stephen Dalton Murine ES cells can be maintained as a pluripotent, self-renewing population by LIF/STAT3-dependent signaling. The downstream effectors of this pathway have not been previously defined. In this report, we...

...Li Li; Esther Arman; Peter Ekblom; David Edgar; Patricia Murray; Peter Lonai This study investigates the establishment of alternative cell fates during embryoid body differentiation when ES cells diverge into two epithelia simulating the pre-gastrulation endoderm and ectoderm. We report...

... in murine embryos and embryonic stem (ES)cells, there have been few in vitro studies of these processes in primates. In this study, we investigated hematopoietic differentiation from cynomolgus monkey ES cells grown on OP9, a stromal cell line deficient in macrophage colony-stimulating factor. Primitive...

...Julia Béjar; Yunhan Hong; Manfred Schartl Embryonic stem (ES) cell lines have provided very useful models to analyse differentiation processes. We present here the development of a differentiation system using ES-like cell lines from medaka. These cells were transfected with the melanocyte...

...Björn Scheffler; Tanja Schmandt; Wolfgang Schröder; Barbara Steinfarz; Leila Husseini; Jörg Wellmer; Gerald Seifert; Khalad Karram; Heinz Beck; Ingmar Blümcke; Otmar D. Wiestler; Christian Steinhäuser; Oliver Brüstle Embryonic stem (ES) cells provide attractive prospects for neural...

...Koji Shimozaki; Kinichi Nakashima; Hitoshi Niwa; Tetsuya Taga Oct3/4 plays a critical role in maintaining embryonic stem cell pluripotency. Regulatable transgene-mediated sustained Oct3/4 expression in ES cells cultured in serum-free LIF-deficient medium caused accelerated differentiation...

... this phase development, we proposed that HNF-4-regulated gene expression in the VE creates an environment capable of supporting gastrulation. To address this directly we have exploited the versatility of embryonic stem (ES) cells which are amenable to genetic manipulation and can be induced to form VE...

... and endocrine cells. * Author for correspondence (e-mail address: [email protected] ) 29 06 1996 © 1996 by Company of Biologists 1996 homologous recombination ES cells W locus c- kit proto-oncogene stem cell factor receptor tyrosine kinase mouse W lacZ / W lacZ mouse...

... and germinal tissue when injected into a recipient embryo. However, these cells were neither identified nor maintained in vitro. Here, we show that chicken early blastoderm contains cells characterised as putative avian embryonic stem (ES) cells that can be maintained in vitro for long-term culture...

... Pax7 homologous recombination mouse neural crest ES cells The mouse Pax gene family consists of nine members (see Gruss and Walther, 1992 for review) which have been isolated on the basis of sequence homology to Drosophila segmentation genes ( Bopp et al., 1986 ; Coté et al., 1987...

...Carolyn H. Buzin; Jeffrey R. Mann; Judith Singer-Sam ABSTRACT We have investigated expression of the Xist gene in mouse female adult kidney, embryos and embryonic stem (ES) cells undergoing in vitro differentiation as embryoid bodies. Using the quantitative RT-PCR single nucleotide primer extension...

...Brian G. Condie; Mario R. Capecchi ABSTRACT Gene targeting in embryo-derived stem (ES) cells was used to generate mice with a disruption in the homeobox-containing gene Hoxd-3 ( Hox-4 . 1 ). Mice homozygous for this mutation show a radically remodeled craniocervical joint. The anterior arch...