... to prefigure the adult. Furthermore, eCNm are transcriptionally divergent from cells in the other nuclei by embryonic day 14.5. We previously showed that loss of the homeobox genes En1 and En2 leads to loss of approximately half of the embryonic eCNm. We demonstrate that mutation of En1/2 in the embryonic eCNm...

... are candidate regulators of this process in the dorsal midbrain (tectum) and anterior hindbrain (cerebellum). En1 mutants lack most of the tectum and cerebellum and die at birth, whereas En2 mutants are viable with a smaller cerebellum and foliation defects. Our previous studies indicated that the difference...

... as critical regulators of this process. Here, we show that the homeoprotein engrailed 1(EN1) is expressed during embryonic and perinatal craniofacial bone development, where it localizes to the skeletogenic mesenchyme, and,subsequently, to calvarial osteoblasts and osteoprogenitors. Mice lacking En1 exhibit...

... or permissive environment produced by the midline tissues or/and a repellent or inadequate environment by the lateral tissues. By contrast, the dorsolateral dermal cells just delaminate from the surface of the intervening space, which expresses En1. Excision of the axial organs or the ectoderm, and grafting...

... 2002. 2002 Emx1 Dlx2 En1 Dlx5 Neurosphere Mouse Cell fate The vertebrate brain is divided into specialized regions during development. A prevailing model proposes that regional specialization in the vertebrate brain is broadly established through the formation of transient...

...Eiji Matsunaga; Isato Araki; Harukazu Nakamura ABSTRACT Transcriptional factors and signaling molecules are responsible for regionalization of the central nervous system. In the early stage of neural development, Pax6 is expressed in the prosencephalon, while En1 and Pax2 are expressed...

.... These morphogenetic effects were apparently due to FGF8-mediated changes in gene expression in the vicinity of the bead, including a repressive effect on Otx2 and an inductive effect on En1, Fgf8 and Wnt1 expression. The ectopic Fgf8 and Wnt1 expression domains formed nearly complete concentric rings around the FGF8...

... factors in regulating these processes in the avian embryo. We show that Fgf4 is expressed in the notochord anterior to Hensen’s node before transcripts for the earliest molecular marker of midbrain tissue in the avian embryo, En1 , are detected. The presence of notochord is required for the expression...

...Mark C. Hanks; Cynthia A. Loomis; Esther Harris; Chung-Xiang Tong; Lynn Anson-Cartwright; Anna Auerbach; Alexandra Joyner ABSTRACT The Engrailed-1 gene, En1 , a murine homologue of the Drosophila homeobox gene engrailed ( en ), is required for midbrain and cerebellum development and dorsal/ventral...

..., demonstrating multiple interneuron cell types express PAX2. Two ventral populations of PAX2-expressing interneurons in the spinal cord are marked by coexpression of the transcription factors, EN1 and EVX1. Interestingly, the expression domains of PAX2, EN1 and EVX1 in postmitotic neurons correlate closely...

... in the mutant limb buds. Thus, some aspects of mesoderm A-P patterning can occur in the absence of SHH and factors normally expressed in the apical ridge. Intriguingly, mutant limbs rescued by local application of FGF displayed a dorsalized feather pattern. Furthermore, the expression of Wnt7a, Lmx1 and En1...