... E-cadherin expression, partially contributing to endoderm C&E defects. Notably, interfering with RhoA function disrupts spatial actomyosin activation without affecting E-cadherin expression. Collectively, our findings identify crucial cellular processes for pharyngeal endoderm C&E and reveal...

... is required for the contraction of junctions at anterior-posterior cell interfaces. The planar polarity of E-cadherin is compromised and E-cadherin accumulates at tricellular junctions after Src42A knockdown. Furthermore, we show that Src42A acts in concert with Abl kinase, which has also been implicated...

... Summary: This Spotlight article reviews how Suzanne Eaton, a pioneer in the field of developmental tissue mechanics, connected quantitative cell behavior with physical modeling to reveal fundamental insights into epithelial morphogenesis. Drosophila E-cadherin Cell packing geometry Junctional...

.... Endocytic activity gradually reduces as tissue remodeling progresses. This reduced endocytic activity accelerates cell elimination through the regulation of Myosin II subcellular reorganization, junctional E-cadherin levels, and caspase activation. Whereas the increased Myosin II dynamics accelerates cell...

... pupal development. Extrusion of these LECs is not closely preceded by caspase activation, involves a pulsatile medial actomyosin network, and correlates with a developmental time period when mechanical tension and E-cadherin turnover at adherens junctions is particularly high. Our work reveals...

... of the primitive streak (PS) by WNT. In the pluripotent state, E-cadherin (E-CAD) transduces boundary forces to focus WNT signaling to the colony border. Following application of WNT ligand, E-CAD mediates a front or wave of epithelial-to-mesenchymal (EMT) conversion analogous to PS extension in an embryo...

... results in more tightly packed outer bud cells, which display stronger E-cadherin localization, reduced cell motility and decreased dynamics of transient cell separations associated with cleft formation; inner bud cells remain unaffected. Mechanistic analyses using in vitro MDCK cells to mimic outer bud...

.... Our results show that E-cadherin is intensely expressed in germline cysts, and that its decline is necessary for oocyte release from the cyst. However, inhibition of JNK signaling leads to aberrantly enhanced localization of E-cadherin at oocyte-oocyte contact sites. WNT4 expression is upregulated...

...) as a direct NF-κB target gene, loss of which replicates a subset of phenotypes seen in NF-κB-deficient embryos. Lhx2 and Tgfb2 knockout embryos exhibit very similar abnormalities in HF development, including failure of the E-cadherin suppression required for follicle down-growth. We show that TGFβ2 signaling...

... Epithelium Stratification Pdx1 Microlumen Lumen diameter E-cadherin Cdh1 β-catenin Endocrine pMLC Insulin-producing β-cells are crucial for proper blood glucose homeostasis. These cells are of particular interest since diabetes, which affects millions of people worldwide and causes both...

...Elise Houssin; Ulrich Tepass; Patrick Laprise E-cadherin-mediated cell-cell adhesion is fundamental for epithelial tissue morphogenesis, physiology and repair. E-cadherin is a core transmembrane constituent of the zonula adherens (ZA), a belt-like adherens junction located at the apicolateral...

...Ghislain Gillard; Massiullah Shafaq-Zadah; Ophélie Nicolle; Raghida Damaj; Jacques Pécréaux; Grégoire Michaux E-cadherin (E-cad) is the main component of epithelial junctions in multicellular organisms, where it is essential for cell-cell adhesion. The localisation of E-cad is often strongly...

... growth morphology, results from tight cell adhesion mediated through E-cadherin, β-catenin (Ctnnb1) and α-catenin with the actin cytoskeleton. β-catenin is also involved in canonical Wnt signaling, which has also been suggested to control mES cell stemness. Here, we analyze Ctnnb1 -/- mES cells in which...

... trafficking of E-cadherin (E-cad), but how this process can be polarised within individual cells is poorly understood. The Frizzled (Fz)-dependent core planar polarity pathway is a major regulator of polarised cell rearrangements in processes such as gastrulation, and has also been implicated in regulation...

...David E. Metzger; Malgorzata Gasperowicz; Florian Otto; James C. Cross; Gerard Gradwohl; Kenneth S. Zaret Pancreatic β-cells arise from Ngn3 + endocrine progenitors within the trunk epithelium of the embryonic pancreas. The emergence of endocrine cells requires E-cadherin downregulation...

... mesoderm cell protrusions. These protrusions extend deep into the underlying ectoderm epithelium and are associated with high levels of E-cadherin at the germ layer interface. Finally, FGF directs distinct hitherto unrecognised and partially redundant protrusive behaviours during later mesoderm spreading...

... that in mouse embryos completely lacking both maternal and zygotic E-cadherin (cadherin 1), the normal epithelial morphology of outside cells is disrupted, but individual cells still initiate TE- and ICM-like fates. A larger proportion of cells than normal showed expression of TE markers such as Cdx2...

...Masayo Shindo; Housei Wada; Masako Kaido; Minoru Tateno; Toshiro Aigaki; Leo Tsuda; Shigeo Hayashi The downregulation of E-cadherin by Src promotes epithelial to mesenchymal transition and tumorigenesis. However, a simple loss of cell adhesion is not sufficient to explain the diverse developmental...

... homology domains to monitor PtdIns(3,4,5) P 3 synthesis, we show that PI3K is constitutively activated in mouse preimplantation embryos. E-cadherin ligation promotes PtdIns(3,4,5) P 3 synthesis at sites of blastomere adhesion at all cleavage stages. In addition, in culture conditions that promote autocrine...

... specification in the mouse. * Author for correspondence (e-mail: [email protected] ) 13 11 2007 © 2008. 2008 Eomesodermin Nodal Axis formation EMT E-cadherin Endoderm specification Mouse The coordinated activities of multiple signalling pathways...