..., isoform-specific, activation function (AF1). We have developed a dominant-negative mutant EcR (EcR-DN), expressed it in flies with the GAL4/UAS system, and used it to block ecdysone signaling in eight tissues or groups of tissues. Localized EcR-DN arrests ecdysone-dependent development in the target cells...

... function is required for neural differentiation of early Xenopus ectoderm. Microinjection of dominant-negative forms of Sox2 ( dnSox2 ) mRNA inhibits neural differentiation of animal caps caused by attenuation of BMP signals. Expression of dnSox2 in developing embryos suppresses expression of N-CAM...

... encodes the dominant-negative Tbx-c. Overexpression of tbx-c causes expansion of the midline mesoderm and formation of ectopic midline structures at the expense of lateral mesodermal cells. In dominant-negative experiments, the midline mesoderm is reduced with the expansion of lateral mesoderm...

... cannot be substituted for the other. In the wing disc, expression of a dominant-negative, truncated form of SERRATE called BD G , is capable of inhibiting NOTCH activation in the ventral but not the dorsal compartments. We demonstrate that BD G can act as a general antagonist of both SERRATE and DELTA...

...-mail: [email protected] ) 15 03 1996 © 1996 by Company of Biologists 1996 pipsqueak psq BTB dominant-negative Drosophila oogenesis Proper development of the Drosophila early egg chamber is required to generate polarity in the egg and embryo. During...