... Hedgehog-containing cytonemes and identified a role for the Hedgehog deployment protein Dispatched in cytoneme stabilization. We demonstrate that Hedgehog and Dispatched colocalize in cytonemes, and that cholesterol-modified Hedgehog acts through Dispatched to increase cytoneme occurrence. Live imaging...

...Hua Tian; Toyoaki Tenzen; Andrew P. McMahon Genetic analyses in Drosophila have demonstrated that a transmembrane protein Dispatched (Disp) is required for the release of lipid-modified Hedgehog (Hh) protein from Hh secreting cells. Analysis of Disp1 null mutant embryos has demonstrated that Disp1...

... sites of synthesis and subsequently moves through the morphogenetic field. The dispatched gene, which encodes a putative multipass membrane protein, was initially identified in Drosophila and is required in Hh-producing cells, where it facilitates the transport of cholesterol-modified Hh. We report...

... pathway. We recovered multiple new alleles of hh and dispatched ( disp ). In addition, we identified a novel component in the hh pathway, which we name central missing ( cmn ). Loss-of-function mutations in cmn cause similar patterning defects to those caused by hh or dispatched ( disp ) mutations...