.... The consequences of spontaneous DNA damage during development are not clear. Here, we define an approach to determine the effects of DNA damage on cell fate choice. Using single cell transcriptomics, we identified a subpopulation of Dictyostelium cells experiencing spontaneous DNA damage. Damaged cells displayed...

... little understanding of the processes that allow cells to become different. One of the clearest examples of developmental self-organisation is shown by Dictyostelium , with cells segregating into two major fates, stalk and spore, within multicellular aggregates. To characterise the gene expression...

...Shrivani Sriskanthadevan; Yingyue Zhu; Kumararaaj Manoharan; Chunxia Yang; Chi-Hung Siu During development of Dictyostelium , multiple cell types are formed and undergo a coordinated series of morphogenetic movements guided by their adhesive properties and other cellular factors. DdCAD-1...

... results in cell sorting is poorly defined. Here we describe a novel gene ( hfnA ) that provides the first mechanistic link between cell signalling, differential gene expression and cell type-specific sorting in Dictyostelium . HfnA defines a novel group of evolutionarily conserved HECT ubiquitin ligases...

...Pauline Schaap Dictyostelium discoideum belongs to a group of multicellular life forms that can also exist for long periods as single cells. This ability to shift between uni- and multicellularity makes the group ideal for studying the genetic changes that occurred at the crossroads between uni...

...Leung Kim; Joseph Brzostowski; Amit Majithia; Nam-Sihk Lee; Vanessa McMains; Alan R. Kimmel In Dictyostelium , the interaction of secreted cAMP with specific cell surface receptors regulates the activation/de-activation of GSK3, which mediates developmental cell patterning. In addition...

... ) Competing interests statement 15 12 2009 © 2010. Dictyostelium Immediate-early response Live cell imaging Transcription The transcriptional response to extracellular signals is integral to cellular choices of proliferation, stress response and differentiation. Cells...

...Yoko Yamada; Hong Yu Wang; Masashi Fukuzawa; Geoffrey J. Barton; Jeffrey G. Williams CudA, a nuclear protein required for Dictyostelium prespore-specific gene expression, binds in vivo to the promoter of the cotC prespore gene. A 14 nucleotide region of the cotC promoter binds CudA in vitro...

...Tetsuya Muramoto; Jonathan R. Chubb The regulation of the Dictyostelium cell cycle has remained ambiguous owing to difficulties in long-term imaging of motile cells and a lack of markers for defining cell cycle phases. There is controversy over whether cells replicate their DNA during development...

...Thomas Keller; Christopher R. L. Thompson One poorly understood mechanism of developmental patterning involves the intermingled differentiation of different cell types that then sort out to generate pattern. Examples of this are known in nematodes and vertebrates, and in Dictyostelium...

...Tsuyoshi Araki; Judith Langenick; Marianne Gamper; Richard A. Firtel; Jeffrey G. Williams STATc becomes tyrosine phosphorylated and accumulates in the nucleus when Dictyostelium cells are exposed to the prestalk cell inducer Differentiation inducing factor 1 (DIF-1), or are subjected to hyper...

...Christopher M. West; Hanke van der Wel; Zhuo A. Wang Development in multicellular organisms is subject to both environmental and internal signals. In Dictyostelium , starvation induces amoebae to form migratory slugs that translocate from subterranean areas to exposed sites, where they culminate...

...Masashi Fukuzawa; Natasha V. Zhukovskaya; Yoko Yamada; Tsuyoshi Araki; Jeffrey G. Williams PstA and pstO cells are the two major populations in the prestalk region of the Dictyostelium slug and DIF-1 is a low molecular weight signalling molecule that selectively induces pstO cell-specific gene...

...Eryong Huang; Simone L. Blagg; Thomas Keller; Mariko Katoh; Gad Shaulsky; Christopher R. L. Thompson The signalling molecule DIF-1 is required for normal cell fate choice and patterning in Dictyostelium . To understand how these developmental processes are regulated will require knowledge of how...

... 28 10 2005 ©2006. 2006 Dictyostelium bZIP Prestalk DIF-1 Primary patterning of the Dictyostelium slug creates an anterior prestalk region and a posterior prespore region but, in addition to the coherent mass of prestalk cells that comprises the front one-fifth of the slug...

...Christina Schilde; Tsuyoshi Araki; Hazel Williams; Adrian Harwood; Jeffrey G. Williams Glycogen synthase kinase 3 (GSK3) is a central regulator of metazoan development and the Dictyostelium GSK3 homologue, GskA, also controls cellular differentiation. The originally derived gskA -null mutant...

...Christopher R. L. Thompson; Qing Fu; Caroline Buhay; Robert R. Kay; Gad Shaulsky The intermingled differentiation and sorting out of Dictyostelium prestalk-O and prespore cells requires the diffusible signaling molecule DIF-1, and provides an example of a spatial information-independent patterning...

...Natasha V. Zhukovskaya,; Masashi Fukuzawa,; Masatsune Tsujioka; Keith A. Jermyn; Takefumi Kawata; Tomoaki Abe; Marketa Zvelebil; Jeffrey G. Williams Dictyostelium , the only known non-metazoan organism to employ SH2 domain:phosphotyrosine signaling, possesses STATs (signal transducers...

...J. Randall Good; Matthew Cabral; Sujata Sharma; Jun Yang; Nancy Van Driessche; Chad A. Shaw; Gad Shaulsky; Adam Kuspa The tag genes of Dictyostelium are predicted to encode multi-domain proteins consisting of serine protease and ATP-binding cassette transporter domains. We have identified a novel...

... ). Dictyostelium myosin II null mutant can still cap Con A receptors. Proc. Natl. Acad. Sci. USA 94 , 9684 -9686. Aguado-Velasco, C. and Bretscher, M. S. ( 1999 ). Circulation of the plasma membrane in Dictyostelium . Mol. Biol. Cell 10 , 4419 -4427. Beckers, C. J., Block, M. R., Glick, B. S...