..., which also lacks epithelial-to-mesenchymal transition. Proliferation and death of progenitors as well as early patterning of the heart appear normal. However, DiI labelling of second heart field progenitors revealed a defect in the addition of cells to the heart. RNA microarray analysis uncovered a set...

... using endogenous and transgenic markers and on DiI labelling, followed by embryo culture. Progenitor cells in the right or left posterior SHF contribute to the right or left common atrium, respectively. Explant experiments with transgenic embryos,in which the transgene marks the right atrium, show...

... into the lumen of the neural tube just after electroporation. The eggs were sealed and allowed to develop for a further 15-30 hours. At the required stages, embryos were photographed in ovo with a Leica MZFLIII dissecting microscope to record GFP expression and DiI labelling. Subsequently, they were dissected...

... the genes. * Author for correspondence (e-mail: [email protected] ) 18 03 1997 © 1997 by Company of Biologists 1997 fatemap limb development chick embryo apical ectodermal ridge DiI labelling Hoxd-13 Fgf-4 The vertebrate limb is an excellent model for studying...

... migration nerve cell differentiation head activator DiI labelling The freshwater polyp hydra has one of the simplest nervous systems in metazoan animals. It consists of a net of nerve cells ( Hadzi, 1909 ) that extends throughout the animal with higher densities in the head and the foot ( Bode et...

... and the otic vesicle lateral to r5. Focal injections of DiI at the levels of r3 and r5 demonstrated that both of these rhombomeres generated neural crest cells. The segmental distribution of neural crest cells resulted from the DiI-labelled cells that originated in r3 and r5 deviating rostrally or caudally...