... statement The authors declare no competing financial interests. 9 1 2013 © 2013. Published by The Company of Biologists Ltd Clearing reagent Whole mount Retinal axon pathway Immunohistochemistry Fluorescent protein DiI Appreciation of neural circuitry...

... of Fgf8 expression in the ventral head ectoderm of stage 11 chick embryos in the presumptive maxillo-mandibular region ( Shigetani et al., 2000 ). As development proceeds, the Fgf8 expression domain expands and corresponds to the developing maxillary and mandibular prominences. DiI labelling...

... migration in splotch mutant embryos requires the genetic defect in both neural crest cells and their migratory environment. DiI or WGA-Au solution was directly deposited in the vicinity of the neural crest by microinjection using a Leitz micromanipulator on a Wild stereomicroscope ( Chan and Tam,1988...

... ). For assessments of ER morphology, dejellied oocytes were attached to protamine-sulfate-coated specimen dishes and microinjected with the ER-specific probe DiI ( Terasaki and Jaffe,1993 ) before examination with a BioRad MRC-600 confocal microscope( Stricker et al.,1998 ). For these studies, serial z -sections...

...David W. McCauley; Marianne Bronner-Fraser The neural crest is a vertebrate-specific cell population that contributes to the facial skeleton and other derivatives. We have performed focal DiI injection into the cranial neural tube of the developing lamprey in order to follow the migratory pathways...

... functional analysis. (2) Vital dye (DiI) labelling in vitro suggests that epiblast cells arrive in the PGE from anterior parts of the embryonic disc and then move within this area in a complex pattern of posterior, centripetal and anterior directions to form the primitive streak. (3) BrdU labelling shows...

..., USA 05 03 2001 19 04 2001 © 2001 by Company of Biologists 2001 Muscle Morphogenesis DiI DiO Somite Involution Dermomyotome Confocal microscopy Fate mapping Chick During early avian embryogenesis, cells in the epiblast layer migrate through Hensen’s Node...

... stage controlling the axon divergence at the chiasm. * Author for correspondence (e-mail: [email protected] ) 10 04 2000 23 05 2000 © 2000 by Company of Biologists 2000 DiI Axon guidance Retina Retinofugal pathway Midline Mouse During development, retinal...

...Hans-Henning Epperlein; Daniel Meulemans; Marianne Bronner-Fraser; Herbert Steinbeisser; Mark A. J. Selleck ABSTRACT We have examined the ability of normal and heterotopically transplanted neural crest cells to migrate along cranial neural crest pathways in the axolotl using focal DiI injections...

... in chick blastoderm explants . Development 115 , 689 – 694 . 10.1242/dev.115.3.689 Primitive streak Gastrulation Fate map Retrovirus DiI Chick Epiblast Cytokinesis Gastrulation is the morphogenetic event through which the three definitive germ layers, ectoderm, mesoderm...

... factor chick fate mapping DiI DiO confocal microscopy These fate mapping experiments permit conclusions to be made about the source, direction of elongation and relative age of the early primary myotome fibers in forelimb-level somites of the 2-day-old chick embryo. These myotome fibers give...

... blastocyst bilateral symmetry bilateral axis polar bodies animal-vegetal axis zygote intercellular bridges midbodies clonal analysis ionic coupling dye coupling lineage labelling embryonic-abembryonic axis DiI fluorescein complexon Spatial patterning of the egg has been implicated...

... for correspondence: email [email protected] 02 05 1995 © 1995 by Company of Biologists 1995 Xenopus heterochronic transplant retinotectal development DiI In the retinotectal system, retinal ganglion cell axons terminate in the optic tectum in a highly ordered pattern. Dorsal...

...Yohko Hatada; Claudio D. Stern ABSTRACT We have used carbocyanine dyes (DiI and DiO) to generate fate maps for the epiblast layer of the chick embryo between stage X and the early primitive streak stage (stages 2 –3). The overall distribution of presumptive cell types in these maps is similar...

...Torsten Bossing; Gerhard M. Technau ABSTRACT We present a new method for marking single cells and tracing their development through embryogenesis. Cells are labelled with a lipophilic fluorescent tracer (DiI) in their normal positions without impaling their membranes. The dye does not diffuse...

... techniques were used to label cells shortly after rhombomeres form (stage 9+ to 13) so that the resultant clones could be followed up to stage 25. Either small groups of cells were labelled with the lipophilic dye DiI or single cells were injected intracellularly with fluorescent dextran. The majority...

... of manipulation and relative transparency of Xenopus laevis embryos to follow neural crest cell migration and differentiation in living embryos. We use two techniques to study the lineage and migratory patterns of frog neural crest cells: (1) injections of DiI or lysinated rhodamine dextran (LRD) into small...

... cells were labelled by injecting DiI into the amniotic cavity on embryonic day 8. Embryos, allowed to develop an additional 1 to 5 days exo utero in the mother before analysis, showed distinct and characteristic patterns of cranial neural crest cell migration at the different axial levels. Neural crest...