...Gavin J. Wright; François Giudicelli; Cristian Soza-Ried; Anja Hanisch; Linda Ariza-McNaughton; Julian Lewis We describe the production and characterisation of two monoclonal antibodies, zdc2 and zdd2, directed against the zebrafish Notch ligands DeltaC and DeltaD, respectively. We use our...

...-mediated endocytosis determines the normal subcellular localization of DeltaD. Knockdown of Notch increases cell surface DeltaA and DeltaD, but not DeltaC, suggesting that,like Mib1, Notch regulates endocytosis of specific ligands. Transplant experiments show that the interaction with Notch, both...

... signalling in the gut stem-cell system and establish the zebrafish as a model for study of the mechanisms controlling renewal of gut epithelium. * Author for correspondence (e-mail: [email protected] ) 15 12 2004 ©2005. 2005 Intestine DeltaD DeltaC Notch mind bomb...

... possess a subset of Delta proteins with a conserved ATEV * motif at their C termini. These ATEV Deltas include Delta1 and Delta4 in mammals and DeltaD and DeltaC in the zebrafish. We show that these Deltas associate with the membrane-associated scaffolding proteins MAGI1, MAGI2 and MAGI3, through a direct...