... via captopril or RNA interference promoted dauer larvae formation, suggesting that acn-1 is a daf gene. Captopril-mediated lifespan extension was abrogated by daf-16(lf) and daf-12(lf) mutations. Our results indicate that captopril and acn-1 influence lifespan by modulating dauer formation pathways...

...Catherine O'Keeffe; Iva Greenwald ABSTRACT Caenorhabditis elegans larvae display developmental plasticity in response to environmental conditions: in adverse conditions, second-stage larvae enter a reversible, long-lived dauer stage instead of proceeding to reproductive adulthood. Dauer entry...

... the arrested state. Although a steroid hormone pathway regulates the entry decision into C. elegans dauer diapause, its role in the exit decision is less clear. A complication to understanding steroid hormonal regulation of dauer has been the peculiar fact that steroid hormone mutants such as daf-9 form...

...Agnieszka Podraza-Farhanieh; Balasubramanian Natarajan; Dorota Raj; Gautam Kao; Peter Naredi ABSTRACT Insulin/IGF signaling in Caenorhabditis elegans is crucial for proper development of the dauer larva and growth control. Mutants disturbing insulin processing, secretion and downstream signaling...

...Colin E. Delaney; Albert T. Chen; Jacqueline V. Graniel; Kathleen J. Dumas; Patrick J. Hu Animals change developmental fates in response to external cues. In the nematode Caenorhabditis elegans , unfavorable environmental conditions induce a state of diapause known as dauer by inhibiting...

... conditions, developing larvae enter a stress-resistant, quiescent stage called ‘dauer’. Dauer larvae are characterized by the arrest of all progenitor cell lineages at a stage equivalent to the end of the second larval stage (L2). If dauer larvae encounter conditions favorable for resumption of reproductive...

..., but the basis for the fertility defect had not been previously characterized. We found that IIR signaling is required for robust larval germline proliferation, separate from its well-characterized role in preventing dauer entry. IIR signaling stimulates the larval germline cell cycle. This activity is distinct...

... in neuronal function in postmitotic,differentiated neurons. Here, we demonstrate a novel role for the canonical Notch signalling pathway in postmitotic neurons during a specialised`diapause-like' post-embryonic developmental stage in C. elegans called dauer. Our data suggest that cell signalling downstream...

...Dhaval S. Patel; Lily L. Fang; Danika K. Svy; Gary Ruvkun; Weiqing Li In C. elegans , steroid hormones function in conjunction with insulin/IGF-1-like signaling in promoting reproductive development over entry into the diapausal dauer stage. The NCR-1 and -2 (NPC1-related) intracellular cholesterol...

... cycle quiescence in the germline stem cell population, in addition to prolonging lifespan. The inactivation of either protein causes aberrant germline proliferation during diapause-like `dauer' development, whereas the loss of AMPK uncouples developmental arrest from lifespan extension. Reduced TGF-β...

... exact function remains unclear. The C. elegans genome contains two homologs of NPC1, ncr-1 and ncr-2 , and an ncr-2;ncr-1 double deletion mutant forms dauer larvae constitutively (Daf-c). We have analyzed the phenotypes of ncr single and double mutants in detail, and determined the ncr gene expression...

...Birgit Gerisch; Adam Antebi In response to the environment, the nematode C. elegans must choose between arrest at a long-lived alternate third larval stage, the dauer diapause, or reproductive development. This decision may ultimately be mediated by daf-9 , a cytochrome P450 related...

...Ho Yi Mak; Gary Ruvkun Parallel pathways control C. elegans reproductive development in response to environmental cues. Attenuation of daf-2 insulin-like or daf-7 TGFβ-like signaling pathways cause developmental arrest at the stress resistant and long-lived dauer stage. Loss-of-function mutations...

...Li S. da Graca; Karen K. Zimmerman; Melissa C. Mitchell; Marianne Kozhan-Gorodetska; Kamila Sekiewicz; Yairani Morales; Garth I. Patterson,, An unconventional TGFβ superfamily pathway plays a crucial role in the decision between dauer diapause and reproductive growth. We have studied the daf-5 gene...

...John Wang; Stuart K. Kim The dauer is a developmental stage in C. elegans that exhibits increased longevity, stress resistance, nictation and altered metabolism compared with normal worms. We have used DNA microarrays to profile gene expression differences during the transition from the dauer state...

...Cathy V. Gunther; Laura L. Georgi; Donald L. Riddle ABSTRACT The daf-4 gene encodes a type II bone morphogenetic protein receptor in Caenorhabditis elegans that regulates dauer larva formation, body size and male tail patterning. The putative type I receptor partner for DAF-4 in regulating dauer...

... and DNase1 protection assays. Mutation of any base in the sequence GTCTG interfered with binding in the gel mobility shift assay, demonstrating that this pentanucleotide is a core recognition sequence for DAF-3 binding. daf-3 is known to promote formation of dauer larvae and this activity is negatively...