... wave DamID Japan Society for the Promotion of Science http://dx.doi.org/10.13039/501100001691 JP21K06196 JP19K23739 JP24K09465 Japan-Taiwan Exchange Association 33063 Ibaraki University 2019 2022 Inamori Foundation http://dx.doi.org/10.13039...

... is poorly understood. Here, we introduce Targeted DamID (TaDa) in C. elegans as a method for identifying genes expressed within a tissue of interest without cell isolation. We show that TaDa signal enrichment profiles can be used to identify genes transcribed in the epidermis and use this method to resolve...

... identification (DamID) profiles genome-wide protein-DNA interactions without antibodies, fixation or protein pull-downs. Recently, DamID has been adapted for applications beyond simple assaying of protein-DNA interactions, such as for studying RNA-chromatin interactions, chromatin accessibility and long-range...

..., France) containing kibra exons 5 to 9 fused to T2A-GFPnls into nos-phiC31Int;MiMIC kibra MI13703 . The Sav reporter constructs were generated by fusing a T2A::GFPnls cassette to the Sav C-terminus. The Sav-coding region and the 3′ UTR segments were then excised and deletions introduced to the Zfh1 DamID...

...Jose Arturo Gutierrez-Triana; Juan L. Mateo; David Ibberson; Soojin Ryu; Joachim Wittbrodt DNA adenine methyltransferase identification (DamID) has emerged as an alternative method to profile protein-DNA interactions; however, critical issues limit its widespread applicability. Here, we present...

... melanogaster . Using a modified DamID approach, we identified 650 DSX-binding regions in the genome from which we then extracted an optimal palindromic 13 bp DSX-binding sequence. This sequence is functional in vivo, and the base identity at each position is important for DSX binding in vitro. In addition...