... morphogenetic protein Dpp in the developing fly wing and that this is necessary for developmental signaling. Inhibition of Irk channels decreases the incidence of distinct Dpp-GFP release events above baseline fluorescence while leading to a broader distribution of Dpp-GFP. Work by others in different cell...

..., such as the Dpp (a Drosophila BMP) pathway. Moreover, Lgd physically interacts with Shrub, a fundamental component of the ESCRT trafficking machinery, whose loss of function results in the activation of several signalling pathways. Here, we show that during oogenesis lgd loss of function causes ectopic activation...

... and patterning are not well understood. In the Drosophila wing imaginal disc, the morphogen Dpp guides patterning and is also required for tissue growth. In particular, it was recently reported that cell division in the disc correlates with the temporal increase in Dpp signaling. Here we mathematically model...

... to those found when signaling through a Drosophila BMP homolog, Decapentaplegic (Dpp), is disrupted. To determine whether Irk2 plays a role in the Dpp pathway, we generated flies in which both Irk2 and Dpp functions are reduced. Irk2DN phenotypes are enhanced by decreased Dpp signaling. In wild-type flies...

... and other tissues, and can diffuse into the pupal wing via the hemolymph. Cv-d binds to the BMPs Dpp and Gbb through its Vg domain, and to heparan sulfate proteoglycans, which are well-known for their role in BMP movement and accumulation in the wing. Cv-d acts over a long range in vivo, and does not have...

...Milán Szuperák; Sally Salah; Emily J. Meyer; Usha Nagarajan; Aissam Ikmi; Matthew C. Gibson The cellular response to the Drosophila BMP 2/4-like ligand Decapentaplegic (DPP) serves as one of the best-studied models for understanding the long-range control of tissue growth and pattern formation...

..., and this specifies the orientation of the D-V axis ( Roth, 2003 ; van Eeden and St Johnston, 1999 ). Through many subsequent steps, the domain of dpp transcription is determined on the dorsal side of the blastoderm embryo ( Morisato and Anderson, 1995 ). The corresponding symmetry-breaking event in Achaearanea...

... and pattern. Thus, the surviving cells have to undergo additional proliferation to compensate for the cell loss. The finding that apoptotic cells ectopically express dpp and wg suggested that ectopic Dpp/Wg signalling might be responsible for compensatory proliferation. We have tested this hypothesis...

... the concentration of the niche-provided self-renewal factor BMP/Dpp in metabolically active high dMyc stem cells. Genetic manipulations that impose uniform dMyc levels across the germline produce an extended Dpp signaling domain and cause uncoordinated differentiation events. We propose that dMyc-induced...

...Gerald Schwank; Simon Restrepo; Konrad Basler Morphogens can control organ development by regulating patterning as well as growth. Here we use the model system of the Drosophila wing imaginal disc to address how the patterning signal Decapentaplegic (Dpp)regulates cell proliferation. Contrary...

... for correspondence (e-mail: [email protected] ) 6 1 2008 © 2008. 2008 Heparan sulfate proteoglycans HSPG Glycosaminoglycan GAG Bone morphogenetic protein signaling Drosophila embryonic patterning Decapentaplegic Dpp Hedgehog Wingless Heparan sulfate proteoglycans (HSPGs...

..., the expanded amnion and serosa anlage correlates with a broader domain of Dpp signaling as compared with the D. melanogaster embryo. Evidence is presented that this expanded signaling is due to altered expression of the sog gene. * Author for correspondence (e-mail: [email protected] ) 3 4 2007...

... in Table 1. In addition to suppression of activated BMP phenotypes, flies heterozygous for the nmo P hypomorphic mutation showed an enhancement in the penetrance of the dpp>tkv QD bifurcated wing phenotype from 20.8% to 86.3%. This finding demonstrates that reduction of nmo can lead...

... characterized the primary sequence and secondary structural requirements for the HOW response element (HRE), and show that this site is necessary and sufficient for HOW binding. Based on this analysis, we have identified the Drosophila TGFβ homolog, dpp , as a novel direct target for HOW negative regulation...

...Julia B. Cordero; David E. Larson; Caroline R. Craig; Rebecca Hays; Ross Cagan The correct organization of cells within an epithelium is essential for proper tissue and organ morphogenesis. The role of Decapentaplegic/Bone morphogenetic protein (Dpp/BMP) signaling in cellular morphogenesis during...

...),synergistically reinforces the weak capacity of either gene, when overexpressed singly, to induce ectopic notum-like development. Whereas the Iro-C genes are activated in the notum anlage by EGFR signalling, tup is positively regulated by Dpp signalling. Our data support a model in which the EGFR and Dpp...

...Erdem Bangi; Kristi Wharton Wing patterning in Drosophila requires a Bmp activity gradient created by two Bmp ligands, Gbb and Dpp, and two Bmp type I receptors, Sax and Tkv. Gbb provides long-range signaling, while Dpp signals preferentially to cells near its source along the anteroposterior (AP...

...Yasuko Akiyama-Oda; Hiroki Oda The mechanism by which Decapentaplegic (Dpp) and its antagonist Short gastrulation (Sog) specify the dorsoventral pattern in Drosophila embryos has been proposed to have a common origin with the mechanism that organizes the body axis in the vertebrate embryo. However...

... nuclear signaling. Ectopic expression of Msk overcomes this block and disrupts patterning. Additionally,the MAPK cytoplasmic hold is genetically dependent on the presence of Decapentaplegic (Dpp) and Hedgehog receptors. † Author for correspondence (e-mail: [email protected] ) * Present address...

... from the R1-R6 neurons stop between two layers of glial cells in the lamina,the most superficial ganglion in the optic lobe. Although it has been suggested that the lamina glia serve as intermediate targets of R axons,little is known about the mechanisms by which these cells develop. We show that DPP...