... highly characterized floral homeotic proteins containing MADS domains, AP2 has two DNA-binding domains referred to as the AP2 domains and its DNA recognition sequence is still unknown. Here, we show that the second AP2 domain in AP2 binds a non-canonical AT-rich target sequence, and, using a GUS reporter...

... DNA. We found that the mutation causes neither a dominant-negative effect nor a gain of function, but instead impairs DNA binding at some sites bound by wild-type HOXD13. Using retrovirus-mediated misexpression in developing chick limbs, we showed that wild-type HOXD13 could upregulate chick EphA7...

... within the dimeric complex to form a bipartite DNA binding domain that recognizes a DNA consensus sequence known as an E-box. eHAND and dHAND (also known as HAND1 and HAND2) are closely related bHLH proteins that control cardiac, craniofacial and limb development. Within the developing limb, dHAND...

... that Drosophila Mad and Medea, and vertebrate Smad3 and Smad4 bind DNA directly ( Kim et al., 1997 ; Yingling et al., 1997 ; Zawel et al., 1998 ; Xu et al., 1998 ). Our in vitro DNA-binding assays used a N-terminal fragment of DAF-3 containing the conserved MH1 domain. Thus, like Drosophila Mad ( Kim et al...

...Susie Jun; Claude Desplan ABSTRACT The Pax proteins are a family of transcriptional regulators involved in many developmental processes in all higher eukaryotes. They are characterized by the presence of a paired domain (PD), a bipartite DNA binding domain composed of two helix-turn-helix (HTH...

... within a hunchback enhancer element. A less than 4-fold increase in Bicoid concentration is sufficient to achieve an unbound/bound transition in DNA binding. Using various biochemical and genetic methods we further demonstrate that Bicoid molecules can interact with each other. Our results are consistent...

... proteins at hundreds of sites along the male polytene X chromosome and that this binding requires the other three MSL proteins. msl-2 encodes a protein with a putative DNA-binding domain: the RING finger. MSL-2 protein is not produced in females and sequences in both the 5′ and 3′ UTRs are important...

... on the spatial patterning of SP60-lacZ expression. * Author for correspondence 18 07 1991 © 1991 by Company of Biologists 1991 Dictyostelium gene regulation cAMP cis- elements trans -factors DNA binding Upon starvation, Dictyostelium vegetative amoebae initiate a 24 h...