...Tsuyoshi Araki; Judith Langenick; Marianne Gamper; Richard A. Firtel; Jeffrey G. Williams STATc becomes tyrosine phosphorylated and accumulates in the nucleus when Dictyostelium cells are exposed to the prestalk cell inducer Differentiation inducing factor 1 (DIF-1), or are subjected to hyper...

...Masashi Fukuzawa; Natasha V. Zhukovskaya; Yoko Yamada; Tsuyoshi Araki; Jeffrey G. Williams PstA and pstO cells are the two major populations in the prestalk region of the Dictyostelium slug and DIF-1 is a low molecular weight signalling molecule that selectively induces pstO cell-specific gene...

...Eryong Huang; Simone L. Blagg; Thomas Keller; Mariko Katoh; Gad Shaulsky; Christopher R. L. Thompson The signalling molecule DIF-1 is required for normal cell fate choice and patterning in Dictyostelium . To understand how these developmental processes are regulated will require knowledge of how...

...Natasha V. Zhukovskaya; Masashi Fukuzawa; Yoko Yamada; Tsuyoshi Araki; Jeffrey G. Williams The ecmA gene is specifically expressed in prestalk cells and its transcription is induced by the chlorinated hexaphenone DIF-1. We have purified a novel bZIP transcription factor, DimB, by affinity...

...-inducing action of DIF-1 but largely refractory to the repressive effect exerted by extracellular cAMP. During normal development, apically situated prestalk cells express the ecmB gene just as they commit themselves to stalk cell differentiation. In the Ax2/gskA– mutant, ecmB is expressed throughout...

...Christopher R. L. Thompson; Qing Fu; Caroline Buhay; Robert R. Kay; Gad Shaulsky The intermingled differentiation and sorting out of Dictyostelium prestalk-O and prespore cells requires the diffusible signaling molecule DIF-1, and provides an example of a spatial information-independent patterning...

...Robert R. Kay; Christopher R. L. Thompson To investigate how cell type proportions are regulated during Dictyostelium development, we have attempted to find out which cell type produces DIF-1, a diffusible signal molecule inducing the differentiation of prestalk-O cells. DIF-1 is a chlorinated...

... of two diffusible signals, cAMP and DIF-1. Cells at different stages of normal development were transferred to shaken suspension, challenged with various combinations of signal molecules and the expression of cell-type-specific mRNA markers measured 1 –2 h later. pDd63, pDd56 and D19 mRNAs were used...

.... All those discovered to date are low MW diffusible compounds: cAMP, adenosine, NH 3 and DIFs 1-3. The DIFs are assayed by their ability to induce isolated amoebae to differentiate into stalk cells and have been identified as a family of chlorinated phenyl alkanones. The diversification of amoebae...