... at the vegetal cortex of Xenopus laevis oocytes. We found that the organization of the cytokeratin filaments but not the actin cytoskeleton depends on the presence of intact VegT mRNA and a noncoding RNA, Xlsirts. Destruction of either of these transcripts results in disruption of the cytokeratin cytoskeleton...

...Janet Heasman; Nicholas Torpey; Chris Wylie ABSTRACT The effects of depleting a maternal cytokeratin mRNA on the developing embryo are described. Cytokeratins are members of the intermediate filament family of cytoskeletal proteins, and are expressed in a cortical network of the superficial...

..., University of Edinburgh, Teviot Place, Edinburgh, EH8 9AG, UK 14 11 1989 © 1990 by Company of Biologists 1990 Kidney development epithelial morphogenesis desmosomes cell-cell adhesion intercellular junctions cytokeratin immunocytochemistry Each kidney of the mouse contains...

... in trunk ectoderm than in head ectoderm. Sequence analysis of one of these clones revealed a high degree of similarity to members of the type II subfamily of intermediate filament cytokeratins. This clone (pCKsel) was used to examine cytokeratin gene expression in ectodermal tissues. A large increase...

...P. Kurki; A. Laasonen; E. M. Tan; E. Lehtonen ABSTRACT A double immunofluorescence method was developed for the monitoring of proliferation and differentiation of F9 embryonal carcinoma cells. Cytokeratin filament expression was used as a marker for differentiation, and proliferating cell nuclear...

...Bernadette Fouquet; Harald Herrmann; Jürgen K. Franz; Werner W. Franke A Xenopus laevis mRNA encoding a cytokeratin of the basic (type II) subfamily that is expressed in postgastrulation embryos was cDNA-cloned and sequenced. Comparison of the deduced amino acid sequence of this polypeptide (513...

...Julia C. Chisholm; Evelyn Houliston ABSTRACT The timing, spatial distribution and control of cytokeratin assembly during mouse early development has been studied using a monoclonal antibody, TROMA-1, which recognizes a 55 000 M r trophecto-dermal cytokeratin (ENDO A). This protein was first...

... labelling using three cytokeratin monoclonal antibodies, for which the polypeptide specificities towards guinea pig cyto-keratins were determined. All the epithelial elements of the adult gland were positively labelled with two monoclonal antibodies, namely KG 8.13 (‘broad spectrum’ anti-cytokeratin...

... by the OCS-1 antibody is apparently present in, or closely associated with, cytokeratin filaments. In addition to mouse oocytes and early embryos, a wide variety of epithelial cells in various species seem to share this antigen(s). The present results suggest that at the early stages, the cytokeratin-related...

..., and may be lineage related. Copyright © 1985 by Company of Biologists 1985 oocyte oogenesis actin tubulin vimentin cytokeratin cytoplasmic determinants germ plasm Xenopus laevis It is clear from many experiments on diverse animal species that regional and cell lineage...