... hedgehog Osteoblasts Calcification Basal epidermis Basement membrane Smoothened inhibitor Cyclopamine BMS-833923 National Institutes of Health 10.13039/100000002 5T32GM007413 National Institutes of Health 10.13039/100000002 1R01HL115294 National Heart, Lung...

... and Whitfield, 2009 ). ptc1 –/– ; lep –/– double-mutant embryos were sorted from siblings at 13-14S based on somite phenotype ( Koudijs et al., 2008 ). Ten to 15 embryos were treated in each well of a 12-well culture dish in 2 ml of embryo medium containing 0.25-50 μM cyclopamine/1% ethanol...

... of Hedgehog signaling by direct binding of cyclopamine to Smoothened. Genes Dev 16 , 2743 -2748. Chen, J. N., Haffter, P., Odenthal, J., Vogelsang, E., Brand,M., van Eeden, F. J., Furutani-Seiki, M., Granato, M., Hammerschmidt, M.,Heisenberg, C. P. et al. ( 1996 ). Mutations affecting...

... Biology, Karolinska Institute, Box 240, S-17177 Stockholm,Sweden ‡ Present address: NIOB/KNAW Hubrecht Laboratorium, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands 5 7 2006 © 2006. 2006 BMP antagonist Cyclopamine Feedback signalling FGF Gremlin1 Hox gene expression Limb...

... † Present address: Biology Department, Williams College, Williamstown, MA 01267, USA 3 6 2005 ©2005. 2005 Zebrafish Cyclopamine Morpholino Axon guidance POC Chiasm Gfap Glia hedgehog slit you-too In bilateral organisms, some axons cross the midline of the CNS...

... the spinal cord but is also transmitted to the surrounding blastema. Cyclopamine treatment revealed that hedgehog signaling is not only required for DV patterning of the regenerating spinal cord but also had profound effects on the regeneration of surrounding, mesodermal tissues. Proliferation of tail...

... obtained from igu +/– incrosses. Cyclopamine and forskolin were dissolved in 100% dimethyl sulfoxide (DMSO)at 10 mM and 60.9 mM, respectively. Embryos were dechorinated with pronase at 50% epiboly and placed in 6 ml of embryo medium( Westerfield, 1993 ) containing 50 μM of cyclopamine...

... of neurogenin1 ( ngn1 ), a gene required for determination of DRG precursors. Cell transplantation experiments demonstrate that Hh acts directly on DRG neuron precursors. Blocking Hh pathway activation at later stages of embryogenesis with the steroidal alkaloid, cyclopamine,further reveals that the requirement...

... gli1 expression present in smu/smo mutant embryos is due to Hh signaling that results from maternal Smoothened function, we treated embryos with the alkaloid cyclopamine from the 2-cell stage throughout embryogenesis. Cyclopamine is thought to completely block Hh signaling at the level of Smoothened...

... Cerebellum Sonic hedgehog Proliferation Cyclopamine Medulloblastoma Nmyc Neural precursor TRRAP Mouse Signaling by the hedgehog family of secreted glycoproteins has been highly conserved through evolution ( Ingham and McMahon, 2001 ). Hedgehog activity is required for fundamental processes...

... expect to lose this expression following cyclopamine treatment. While fin buds of cyclopamine-treated embryos fail to express ptc ( Fig. 4H ), they show clear posterior activation of hoxd-11 and hoxd-12 ( Fig. 4I and data not shown), and thus phenocopy syu mutant buds. Taken together, these data...

...John P. Incardona; William Gaffield; Raj P. Kapur; Henk Roelink ABSTRACT The steroidal alkaloid cyclopamine produces cyclopia and holoprosencephaly when administered to gastrulation-stage amniote embryos. Cyclopamine-induced malformations in chick embryos are associated with interruption of Sonic...