... domain was expanded along the proximal-distal axis (mean length=142%, n =5 control and 10 BMS-833923-treated fish, P <0.0001; Fig. 5 K,L), reminiscent of a previous report using cyclopamine ( Quint et al., 2002 ). Collectively, we conclude that: (1) Shha/Smo signaling is not required to generate...

... regulation of Hh activity required. In mouse otic vesicles, ptc1 is expressed in a graded fashion, highest ventromedially ( Bok et al., 2007b ); the expression of dzip1, su ( fu ) and hip has not yet been described in amniote ears. Table 2. Application of cyclopamine to ptc1 –/– ; lep...

... for classical morphogens: a role as graded positional cues in axon guidance. Development 132 , 2251 -2262. Chen, J. K., Taipale, J., Cooper, M. K. and Beachy, P. A. ( 2002 ). Inhibition of Hedgehog signaling by direct binding of cyclopamine to Smoothened. Genes Dev 16 , 2743 -2748. Chen, J. N...

... expression domains of 5′Hoxd genes, SHH signal transduction was blocked at specific time points by culturing limb buds in the presence of the inhibitor cyclopamine( Chen et al., 2002 ). This approach was chosen as the genes of interest are either not expressed or downregulated in limb buds of Shh -deficient...

... † Present address: Biology Department, Williams College, Williamstown, MA 01267, USA 3 6 2005 ©2005. 2005 Zebrafish Cyclopamine Morpholino Axon guidance POC Chiasm Gfap Glia hedgehog slit you-too In bilateral organisms, some axons cross the midline of the CNS...

... the spinal cord but is also transmitted to the surrounding blastema. Cyclopamine treatment revealed that hedgehog signaling is not only required for DV patterning of the regenerating spinal cord but also had profound effects on the regeneration of surrounding, mesodermal tissues. Proliferation of tail...

... cell specification Hedgehog signaling Cyclopamine cAMP-dependent kinase A Forskolin Midline mutant Adaxial cells dzip1 PEST sequence Zebrafish During animal development, cell fates are specified in response to gradients of signaling molecules within the embryo. Members...

... of neurogenin1 ( ngn1 ), a gene required for determination of DRG precursors. Cell transplantation experiments demonstrate that Hh acts directly on DRG neuron precursors. Blocking Hh pathway activation at later stages of embryogenesis with the steroidal alkaloid, cyclopamine,further reveals that the requirement...

... ). Control of cell pattern in the neural tube by the zinc finger transcription factor and oncogene gli-1. Neuron 19 , 15 -26. Incardona, J. P., Gaffield, W., Kapur, R. P. and Roelink, H. ( 1998 ). The teratogenic Veratrum alkaloid cyclopamine inhibits sonic hedgehog signal transduction. Development...

... of Ptch heterozygotes. Nmyc overexpression in vitro promoted CGNP proliferation and cyclin D1 protein upregulation in the presence of the Shh signaling inhibitor cyclopamine. Finally, antagonism of Nmyc activity significantly decreased CGNP proliferation induced by Shh. Together, our data provide...

... to hedgehog . Trends Genet . 13 , 14 – 21 . 10.1016/S0168-9525(96)10051-2 Incardona , J. P. , Gaffield , W. , Kapur , R. P. and Roelink , H. ( 1998 ). The teratogenic Veratrum alkaloid cyclopamine inhibits Sonic hedgehog signal transduction . Development 125 , 3553...

...John P. Incardona; William Gaffield; Raj P. Kapur; Henk Roelink ABSTRACT The steroidal alkaloid cyclopamine produces cyclopia and holoprosencephaly when administered to gastrulation-stage amniote embryos. Cyclopamine-induced malformations in chick embryos are associated with interruption of Sonic...