... of Aberdeen,Aberdeen AB25 2ZD, UK † Present address: MRC Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Headington,Oxford OX3 9DS, UK 10 12 2004 ©2005. 2005 Activin Animal caps Cycloheximide Emetine...

... of new cyclin synthesis is ablated by antisense oligonucleotides, the oocytes degenerate and fail to form a second meiotic spindle. The effects on meiotic progression are even more severe when all new protein synthesis is blocked by cycloheximide added after meiosis I, but can be rescued by injection...

...+ oscillations in response to microinjection of inositol trisphosphate. This was explored further by treating in vitro maturing oocytes at metaphase I for 4-5 hours with cycloheximide, which induced nuclear progression to inter-phase (nucleus formation) and subsequent re-entry to metaphase (nuclear envelope...

..., ix and dsx . We have asked a number of basic questions about how these genes are regulated, using an inhibitor of protein synthesis (cycloheximide), labelling RNA in vivo , a temperature-sensitive sex-determination mutant ( tra-2 tsl ), and 20-hydroxyecdysone. We have found that the yolk-protein...

...S. Cascio; J. B. Gurdon ABSTRACT The incubation of Xenopus embryo fragments in cycloheximide at 5 or 10μgml −1 rapidly inhibits protein synthesis to 10 % or less of control levels. In most batches of embryos, treatment with cycloheximide for up to 1 h causes no obvious cellular damage and protein...

... dependent on new protein synthesis. By delaying the addition of the inhibitor, cycloheximide, for progressively longer periods after the resumption of meiosis it was established that the required synthesis occurs in the 1–2 h immediately preceding germinal vesicle breakdown (GVBD). The action...