... plate, is responsible for G2/M transition in meiotic mouse oocytes. Depletion of CenpH by morpholino injection decreased cyclin B1 levels, resulting in attenuation of maturation-promoting factor (MPF) activation, and severely compromised meiotic resumption. CenpH protects cyclin B1 from destruction...

... oocytes to remain prophase I/germinal vesicle (GV) arrested in vitro was also compromised, despite the addition of the phosphodiesterase milrinone. Meiotic competency of smaller diameter oocytes was also accelerated by Fzr1 loss. Cyclin B1 levels were elevated ~5-fold in Fzr1 Δ/Δ oocytes, whereas securin...

... in the concentrations of the Cdk1-binding partner cyclin B1, a process achieved by anaphase-promoting complex (APC Cdh1 ) activity, which ubiquitylates and so targets cyclin B1 for degradation. Thus, APC Cdh1 activity prevents precocious meiotic entry by promoting cyclin B1 degradation. However, it remains unresolved...

... and multimer assembly. This study used immunoprecipitation-coupled western blot and in vitro reconstitution experiments to show that HSP70-2 interacts with CDC2 in the mouse testis, appears to be a molecular chaperone for CDC2, and is required for CDC2/cyclin B1 complex formation. Previous studies reported...