... and abundantly expressed in the rat invasive trophoblast cell lineage. Disruption of Prl7b1 did not adversely affect placental development. We demonstrated that the Prl7b1 locus could be effectively used to drive the expression of Cre recombinase in invasive trophoblast cells. Our rat model represents a new tool...

... terminal mitosis. FGF8 Hair cell Vestibular Inner ear Cre recombinase Mouse The vestibular sensory organs of the vertebrate inner ear contain mechanosensory receptors called hair cells that enable the detection of linear and rotational accelerations of the head, and gravity. Vestibular...

... through electroporation. (A) Twelve plasmid labelling vectors, collectively named as pPB-STOP-XFPs, carry the expression of one fluoroprotein arrested by a loxP-flanked STOP signal. The targeting vector encodes Cre recombinase under a promoter of our choice. Only progenitor cells expressing the selecting...

... repeats and the PEST and transcriptional activation domain (Tad-PEST). S2 and S3 indicate Adam metalloprotease-dependent cleavage and the γ-secretase dependent cleavage at Val1744, respectively. Using gene-targeting, Cre recombinase was inserted immediately downstream of Val1744 at Arg1752. Interaction...

... Lilly and Company, Indianapolis, IN 46285, USA 23 9 2005 ©2005. 2005 FGF Vomeronasal organ Neurogenesis Olfactory epithelium Nasal cavity Stem cell Apoptosis Cre recombinase Fgf8 Foxg1 Sox2 Pax6 Mash1 Neurogenin 1 Ncam1 Pyst1 Shh Dlx5 Neuronal...

... survival and tubulogenesis. † Author for correspondence (e-mail: [email protected] ) * These authors contributed equally to this work 17 6 2005 ©2005. 2005 Cre recombinase Fgf8 Kidney Lim1 Nephron Somitogenesis T-Cre Wnt4 Mouse Fibroblast...

... map Genetic ablation Bmp Gdf7 Transgenic mice Cre recombinase Diphtheria toxin Mouse The choroid plexus (CP) produces the cerebrospinal fluid (CSF) that surrounds the brain and spinal cord, and fills the ventricular system. The CP and CSF are thought to have multiple functions (e.g...

[email protected] ) * PRESTO, Japan Science and Technology Corporation (JST) 30 6 2003 © 2003. 2003 Mouse Cell lineage Cre recombinase It is well known that the future embryonic body axes are specified prior to the first cleavage in a variety of animals. In the case...

... in skeletal development. Embryos harboring homozygous null mutations in FGFR2 die prior to skeletogenesis. To address the role of FGFR2 in normal bone development, a conditional gene deletion approach was adopted. Homologous introduction of cre recombinase into the Dermo1 ( Twist2 ) gene locus resulted...

... the differentiation process of alveolar precursor cells in response to lactogenic hormones. The transient upregulation of Cre recombinase in differentiating epithelial cells during pregnancy permanently activates a ubiquitously expressed reporter transgene (Rosa- lacZ ), whose expression is not dependent...

... in Krox20 -null embryos, and in embryonic chimaeras between wild-type and Krox20 mutant cells. Besides the Krox20 lacZ allele described above, we made use of a Krox20 Cre allele ( Voiculescu et al., 2000 ), which carries a Cre recombinase gene insertion into the locus. This Krox20 Cre mouse line...

... by expressing the Cre recombinase gene under control of the MMTV-LTR. By the age of five weeks, Bcl-x conditional mutant mice exhibited hyperproliferation of megakaryocytes and a decline in the number of circulating platelets. Three-month-old animals suffered from severe hemolytic anemia, hyperplasia...

[email protected] ) 18 02 1999 06 04 1999 © 2000 by Company of Biologists 2000 Pax3 Cardiogenesis connexin 43 Cre recombinase Neural crest Mouse In birds and mammals the complex process of cardiac morphogenesis requires interactions between multiple cell types intrinsic...

.... Determination of the fate of mammalian neural crest has been inhibited by the lack of appropriate markers. Here, we make use of a two-component genetic system for indelibly marking the progeny of the cranial neural crest during tooth and mandible development. In the first mouse line, Cre recombinase...

... mark the neural crest cell lineage in mice by gene expression. One component is a transgene expressing Cre recombinase under the control of the Wnt1 promoter and enhancer ( Danielian et al., 1998 ). The Wnt1 gene is expressed specifically in the neural plate, in the dorsal neural tube...