... for trophectodermal Tat-Cre/loxP recombination. We used the natural permeability barrier in mouse blastocysts in combination with off-the-shelf Tat-Cre recombinase to achieve editing of conditional alleles in the trophoblast lineage. This direct approach enables gene function analysis during implantation...

...Congde Chen; Chiyo Shiota; Guy Agostinelli; Daniel Ridley; Yinan Jiang; Jie Ma; Krishna Prasadan; Xiangwei Xiao; George K. Gittes ABSTRACT The Cre/loxP system has been used extensively in mouse models with a limitation of one lineage at a time. Differences in function and other properties among...

... line and labeled NC cells by inducing Cre/loxP recombination with tamoxifen at embryonic stages. In juvenile and adult fish, we identified numerous established NC derivatives and, in the cranium, we precisely defined the crest/mesoderm interface of the skull roof. We show the NC origin of the opercular...

... progenitor cells Retinogenesis Crx Cre/ loxP As in other regions of the CNS, neuronal diversity in the retina originates from a common pseudostratified layer of mitotically active neuroepithelium. The retinal neuroepithelium emerges from the distal tip of the optic vesicles(OVs), established...

... and endows them with neurogenic properties. Using a Cre/ loxP -based approach, we studied the effect of conditional activation of two Pax6 isoforms, Pax6 and Pax6-5a ,on the corticogenesis of transgenic mice. We found that activation of either Pax6 or Pax6-5a inhibits progenitor proliferation...

... function directly during retinal neurogenesis by selective Cre/ loxP -triggered Notch1 gene inactivation in peripheral retinal progenitor cells (RPCs) prior to the onset of cell differentiation. Consistent with its previously established role, Notch1 inactivation led to dramatic alteration...

... in the early telencephalon. In particular, Fgf8 is expressed at the anterior end of the telencephalon and is hypothesized to pattern it along the anteroposterior (AP) axis. Using a CRE/ loxP genetic approach to disrupt genes in the telencephalon, we address the role of FGF signaling directly in vivo...

... of a conditional Jun allele by cre/LoxP-mediated recombination was used to screen for novel functions of Jun and revealed that its absence results in severe malformations of the axial skeleton. More-specific Jun deletion by collagen2a1-cre demonstrated the essential function of Jun in the notochord and sclerotome...

... embryonic death. In the present study, we have specifically inactivated this gene in hepatocytes and bile duct cells using the Cre/loxP system. Mutant animals exhibited severe jaundice caused by abnormalities of the gallbladder and intrahepatic bile ducts (IHBD). The paucity of small IHBD was linked...

... mutagenesis Cre/loxP Keratinocytes Epidermis Hair follicle Hair cycle RXR/VDR Heterodimer Mouse The skin is composed of the epidermal layer and its appendages (hair follicles) that are separated from the dermal layer by a basement membrane. The epidermis, a stratified epithelium made...

... of Biologists 2000 Vascular endothelial growth factor (VEGF) Angiogenesis Cartilage Collagen2a1 Cre/loxP Conditional gene targeting Heart development Cardiomyopathy The VEGF-Flk/Flt1 signaling system has been demonstrated to be essential for the proper development of the vascular system...

...Hans-Peter Gerber; Kenneth J. Hillan; Anne M. Ryan; Joe Kowalski; Gilbert-Andre Keller; Linda Rangell; Barbara D. Wright; Freddy Radtke; Michel Aguet; Napoleone Ferrara ABSTRACT We employed two independent approaches to inactivate the angiogenic protein VEGF in newborn mice: inducible, Cre- loxP...