... were removed with a 70 μm nylon cell strainer after all preparation procedures. Remaining cells were washed with PBS and cells analyzed on adhesion slides by confocal microscopy and flow cytometry. Specimens of fetal trunk skin (15-22 weeks EGA) were taken after legal termination of pregnancy...

... behaviors of embryonic tissues during quail body axis elongation. Confocal microscopy Live imaging Quail embryo Axis elongation Proliferation PSM Multi-tissue Tissue deformations Morphogenesis Axis formation in the vertebrate embryo occurs in a head-to-tail sequence; the anterior part...

... of fluorescent proteins and dyes and allows the structural analysis of gene expression patterns in multiple plant tissues. Clearing Whole plant Deep imaging Confocal microscopy Two-photon microscopy Arabidopsis thaliana Physcomitrella patens To understand how cell patterning changes...

... Summary: A novel transgenic quail that ubiquitously expresses nuclear localized CherryFP provides insights into the cellular and morphogenetic events of amniote embryogenesis. Confocal microscopy Quail embryo Live imaging It is easy to overlook that the developing embryo – biochemically...

... ) * These authors contributed equally to this work 14 2 2005 ©2005. 2005 Planarians Regeneration Neoblasts Stem cells RNAi Cell proliferation Pumilio PUF proteins Confocal microscopy TEM Cytofluorimetry In situ hybridization Post-transcriptional regulation Stem cells...

... basis for DML activity. The ablated DML of chick wing-level somites was replaced with tissue fragments from various mesoderm regions of quail embryos and their capacity to form myotomal tissue assessed by confocal microscopy. Transplanted fragments from the epithelial sheet region of the dermomyotome...

... 2001 © 2001 by Company of Biologists 2001 Muscle patterning Chick/quail chimera Somite Dermatome Confocal microscopy Fate mapping The first differentiated skeletal muscle cells to appear during early vertebrate development are those of the epaxial myotome, the segmented...

...) Development 124, 1601-1610), a finding recently substantiated through surgical transplantation studies (C. P. Ordahl, E. Berdougo, S. J. Venters and W. F. Denetclaw, Jr (2001) Development 128, 1731-1744). Confocal microscopy was used here to analyze the location and pattern of myotome cells whose precursors...

...Wilfred F. Denetclaw, Jr; Charles P. Ordahl ABSTRACT Myotome formation in the epaxial and hypaxial domains of thoraco-lumbar somites was analyzed using fluorescent vital dye labeling of dermomyotome cells and cell-fate assessment by confocal microscopy. Muscle precursor cells for the epaxial...

... at the tips of these neuronal processes. Although the behavior of growth cones has been well-characterized in vitro, it is difficult to observe growth cones in vivo. We have observed motor neuron growth cones migrating in living Caenorhabditis elegans larvae using time-lapse confocal microscopy. Specifically...

... at specific sites in the dermomyotome in ovo and the fates of dye-labeled cells monitored by confocal microscopy. Dyelabeled myotome myofibers were generated from cells injected along the entire medial boundary and the medial portion of the cranial boundary of the dermomyotome, regions in close proximity...

... and eukaryotic flagella. * Author for correspondence (e-mail: [email protected] ) 17 01 1996 © 1996 by Company of Biologists 1996 cortical rotation Xenopus microtubule confocal microscopy In many animal species, normal embryonic development depends on the proper positioning...

... and nonradial cells. These results suggest that nonradial cells use strategies and substrata for migration that differ from those employed by radial cells. * Author for correspondence neuronal migration cerebral cortex confocal microscopy radial glia CNS ferret REFERENCES Angevine...

...Philip L. Hertzler; Wallis H. Clark ABSTRACT Embryos of the penaeoidean shrimp Sicyonia ingentis were examined at intervals during cleavage and gastrulation using antibodies to β-tubulin and DNA and laser scanning confocal microscopy. Cleavage occurred in a regular pattern within four domains...

... be addressed 05 12 1991 © 1992 by Company of Biologists 1992 MPF confocal microscopy sperm aster Specification of the dorsal-ventral (D-V) axis of frog embryos occurs early in development, during a critical period between fertilization and first cleavage ( Scharf and Gerhart...