...Jong-Won Lim; Pamela Hummert; Jason C. Mills; Kristen L. Kroll Transient maintenance of a pluripotent embryonic cell population followed by the onset of multi-lineage commitment is a fundamental aspect of development. However, molecular regulation of this transition is not well characterized...

.... Double-mutant animals had defective differentiation and cell fate commitment. The expression levels of markers of all the differentiated cell types, both enterocytes and secretory cells, were affected. In addition, the number of goblet cells was increased, whereas mature Paneth cells were missing...

... commitment just prior to morphological differentiation. * Author for correspondence (e-mail: [email protected] ) 17 10 2008 © 2008. 2008 Olfactory placode Nasal placode Chick Competence Specification Commitment Induction Ectoderm Cranial sensory placodes arise...

... of inducible plasticity is retained by cells of the adult central nervous system. † Author for correspondence (e-mail: [email protected] ) * Co-senior authors 6 7 2006 © 2006. 2006 Astrocyte Progenitor Plasticity Human Expansion Commitment Transplantation...

... specified to form the otic placode at the 4-6ss, and that this ectoderm is committed to a placodal fate by the 10ss. We also demonstrate that much of the embryonic ectoderm is competent to generate an otic placode if taken at a sufficiently early age. We have mapped the location of otic placode-inducing...

... of neurulation in chick embryos. Notochords or Shh-secreting cells are able to prevent neural crest formation at open neural plate levels, as assayed by DiI-labeling and expression of the transcription factor, Slug , suggesting that neural crest cells are not committed to their fate at this time. In contrast...

... for correspondence (e-mail: [email protected] ) 15 10 1998 9 11 1998 © 1998 by Company of Biologists 1998 Potentiation Chromatin structure Protamine Phosphoglycerate kinase Spermatogenesis Determination Commitment Every step along the pathway toward gene expression...

... was transformed into lateral plate tissue, indicating that the primitive somite was not fully committed and that the lateral plate has a cue for mesodermal lateralization. Since the lateral plate expresses a high level of BMP-4 mRNA, a member of the TGF-β family, we hypothesized that it is the molecule...

... for correspondence (e-mail: [email protected] ) 06 12 1996 © 1997 by Company of Biologists 1997 induction cerebral cortex specification commitment pattern formation neuron cortical neuron As the structure responsible for the highest levels of cognitive function...

... motoneurons remains plastic until just prior to axogenesis when they become committed to particular identities. We find that distinct primary motoneurons express particular combinations of LIM homeobox genes. Expression precedes axogenesis as well as commitment, suggesting that LIM homeobox genes may...

...Roger E. Breitbart; Chang-seng Liang; Leslie B. Smoot; Dan A. Laheru; Vijak Mahdavi; Bernardo Nadal-Ginard ABSTRACT The transition from multipotent mesodermal precursor to committed myoblast and its differentiation into a mature myocyte involve molecular events that enable the cell to activate...

... by which cellular identity is conferred and maintained in the zebrafish. In this chapter, I describe single cell transplantation experiments designed to test for the irreversible restriction or ‘commitment’ of embryonic blastomeres in the zebrafish embryo. These experiments support the hypothesis that cell...

... in vivo competition commitment specification gene transfer gene regulation in early development The early blastula of the sea urchin Strongylocentrotus purpuratus consists of five embryonic territories, each composed of a unique set of clones, and each displaying a unique developmental fate...

... cells and tissues, and when these commitments are made. The observed patterns of male and female cells in individual animals indicate that sex determination decisions can be influenced by anterior-posterior position and that sex determination decisions can be made as late as the third larval stage...

...Richard P. Harvey Expression of the mouse MyoD gene appears to represent a critical point in the commitment of cultured cells to muscle. In Xenopus , myogenic commitment begins during mesoderm induction which is initiated early in development by endogenous growth factors. To study MyoD gene...

...Tsuyoshi Noguchi; Hiroaki Fukumoto; Yuji Mishina; Masuo Obinata ABSTRACT On addition of DMSO, the MEL cell line TSA8 becomes committed into erythroid progenitor cells (CFU-E) which can form differentiated colonies in the presence of erythropoietin. To understand the mechanism of cellular commitment...

...Gerhard M. Technau The mechanisms leading to the commitment of a cell to a particular fate or to restrictions in its developmental potencies represent a problem of central importance in developmental biology. Both at the genetic and at the molecular level, studies addressing this topic using...

....82.1.139 Heasman , J. , Snape , A. , Smith , J. C. , Holwill , S. & Wylie , C. C. ( 1985 ). Cell lineage and commitment in early amphibian development . Phil. Trans. R. Soc . B 312 , 145 – 152 . Heasman , J. , Snape , A. , Smith , J. C. & Wylie...

... accurately the time during early embryogenesis at which individual blastomeres become committed. In the vegetal pole, determination towards endoderm is a gradual process beginning during the middle blastula stage (stage 8) and completed by the beginning of gastulation (stage 10). This method offers...

... ). Formation and consequences of cell patterns in preimplantation mouse development . J. Embryol. exp. Morph . 49 , 277 – 294 . Handyside , A. H. ( 1978 ). Time of commitment of inside cells isolated from preimplantation mouse embryos . J. Embryol. exp. Morph . 45 , 37 – 53...