...Akanksha Bhatt; Valentin Ruffine; Uwe Töpfer; Jinhee Ryu; Elisabeth Fischer-Friedrich; Christian Dahmann ABSTRACT The elongation of tissues and organs is important for proper morphogenesis in animal development. In Drosophila ovaries, the elongation of egg chambers involves aligned Collagen IV...

... during early pregnancy. The 3DMOUSEneST method incorporates second-harmonic generation microscopy to image the three-dimensional structure formed by decidual fibrillar collagen, named ‘decidual nest’, and third-harmonic generation microscopy to evaluate early conceptus (defined as the embryo and extra...

... Niche Perlecan Collagen Integrin Laminin National Institutes of Health R21HD103989 R35GM118081 American Cancer Society http://dx.doi.org/10.13039/100000048 PF-16-098-01-DDC Stem cells must balance proliferation and differentiation in order to meet...

... composition and mechanical properties of the basement membrane underlying the follicle epithelium of Drosophila egg chambers. We find that the Laminin and Collagen IV networks largely persist in the absence of the other components. Moreover, we show that Perlecan and Collagen IV, but not Laminin or Nidogen...

... is to secrete collagen-containing bone matrix. However, it remains unclear how the dynamics of collagen trafficking is regulated during skull development. Here, we reveal the regulatory mechanisms of ciliary and golgin proteins required for intramembranous ossification. During normal skull formation...

... that failed to be secreted in both mutants was a type II collagen (Col2a1). Col2a1 is normally secreted by notochord sheath cells, alongside which LECs migrate. col2a1a mutants displayed defects in the migratory behaviour of LECs and failed lymphangiogenesis. These studies thus identify Col2a1 as a key cargo...

... induces fibroblast migration in 3D extracellular matrix (ECM) through regulation of actomyosin cytoskeleton and promotes force-mediated collagen remodeling by mammary fibroblasts. Moreover, FGF2 regulates production of ECM proteins by mammary fibroblasts, including collagens, fibronectin, osteopontin...

... Collagen, Perlecan and Nidogen/entactin (NDG). Recent in vivo studies challenged the initially proposed role of NDG as a major ECM linker molecule by revealing dispensability for viability and BM formation. Here, we report the characterization of the single Ndg gene in Drosophila. Embryonic Ndg expression...

...Richard J. Goodyear; Xiaowei Lu; Michael R. Deans; Guy P. Richardson The tectorial membrane is an extracellular structure of the cochlea. It develops on the surface of the auditory epithelium and contains collagen fibrils embedded in a tectorin-based matrix. The collagen fibrils are oriented...

... at the age of 10 weeks and expression remained at similar levels at 12 months. In Mkx −/− mice, age-dependent expansion of the PDL at the maxillary first molar (M1) furcation area was observed. Transmission electron microscopy (TEM) revealed that Mkx −/− mice presented collagen fibril degeneration in PDL...

... regulating collagen maturation and organization, uncouples the balance between the amount of myofibers and that of muscle connective tissue (MCT). We show that Lox secreted from the myofibers attenuates TGFβ signaling, an inhibitor of myofiber differentiation and promoter of MCT development. We further...

..., and defects in its structure correlate with a subset of muscular dystrophies. Col22a1 encodes the MTJ component collagen XXII, the function of which remains unknown. Here, we have cloned and characterized the zebrafish col22a1 gene and conducted morpholino-based loss-of-function studies in developing embryos...

...Lital Bentovim; Roy Amarilio; Elazar Zelzer Collagen production is fundamental for the ontogeny and the phylogeny of all multicellular organisms. It depends on hydroxylation of proline residues, a reaction that uses molecular oxygen as a substrate. This dependency is expected to limit collagen...

.... 1 , 236 - 248 . Ng L. J. , Wheatley S. , Muscat G. E. , Conway-Campbell J. , Bowles J. , Wright E. , Bell D. M. , Tam P. P. , Cheah K. S. , Koopman P. ( 1997 ). SOX9 binds DNA, activates transcription, and coexpresses with type II collagen...

... cloning revealed that stumpy was the zebrafish homolog of the atypical FACIT collagen collagenXIXa1 ( colXIX ). colXIX expression was observed in a temporal and spatial pattern, consistent with a role in motor axon guidance at intermediate targets. Knocking down zebrafish ColXIX phenocopied the stumpy...

... in trabecular bone occur independently of the improper cartilage ECM degradation caused by Mmp13 deficiency in late hypertrophic chondrocytes. Our studies identified the two major components of the cartilage ECM, collagen type II and aggrecan, as in vivo substrates for MMP13. We found that degradation...

... have established the general molecular structure of hydra ECM and indicate that it is organized as two subepithelial zones that contain basement membrane components such as laminin and a central fibrous zone that contains interstitial matrix components such as a unique type I fibrillar collagen...

... little about the nature of hydra collagens. In the current study we provide a molecular, biochemical and functional analysis of a hydra fibrillar collagen that has similarity to vertebrate type I and type II collagens. This fibrillar collagen has been named hydra collagen-I (Hcol-I) because of its...

... procollagen IIA (200 pg/injection). VMZ explants were excised at early gastrula and cultured until stage (G) RT-PCR analysis of VMZ explants treated as above; the expression of the dorsal marker α -actin and EF-1α were analyzed by RT-PCR. Note that full-length collagen, but not the CR domain alone, can...

... by a pathway of heterochronic genes that includes the relatively direct regulatory gene, lin-29 , and upstream genes lin-4, lin-14 and lin-28 . Using northern analysis to detect endogenous collagen mRNA levels and collagen/lacZ reporter constructs to monitor collagen transcriptional activity, we show...