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Keywords: Clonal analysis
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Journal Articles
Journal: Development
Development (2021) 148 (7): dev193565.
Published: 15 April 2021
...-RIA-CRISPR and RIA-H2B-RFP retrovirus were mixed at a volumetric ratio of 10:1 and injected into the lumen of the neural tube in chick embryos at HH12. Then, 72 h post injection, embryos were fixed with 4% paraformaldehyde (PFA) in PBS for 30 min at 4°C. To perform clonal analysis, the trunk neural...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2020) 147 (20): dev193193.
Published: 23 October 2020
... studies, and how clonal analysis can be applied to questions regarding multipotency. We also highlight how effective progenitor cell tracing, when combined with recently developed molecular and imaging tools, such as single-cell transcriptomics, single-molecule fluorescence in situ hybridization and high...
Journal Articles
Journal: Development
Development (2019) 146 (1): dev170969.
Published: 04 January 2019
... range of experimental settings, for example to modulate clone frequency with existing tools in established model organisms and to introduce clonal analysis in emerging experimental models. * Authors for correspondence ( anna.gilles@trigenes.com ; johannes.schinko@trigenes.com ; michalis.averof...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2018) 145 (13): dev157222.
Published: 02 July 2018
... from a simple bilayer into a multilayered embryo enveloped by its extra-embryonic membranes. Impaired development of the amnion, the innermost membrane, causes major malformations. To clarify the origin of the mouse amnion, we used single-cell labelling and clonal analysis. We identified four clone...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2017) 144 (16): 2889–2895.
Published: 15 August 2017
... cells is restricted to the joints of uninjured fins but contributes more broadly during regeneration. Clonal analysis Fin regeneration Regeneration Zebrafish Zebrafish vigorously regenerate fins after an amputation injury, an analogous event to limb regeneration in salamanders ( Tornini...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2016) 143 (10): 1688–1696.
Published: 15 May 2016
... ventricular morphogenesis at the cellular level. Atrium Brainbow Cardiomyocyte Clonal analysis Heart development Zebrafish The heart achieves its form through the specification, proliferation and organization of cardiac muscle cells and their supporting tissues. Congenital defects in these...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2016) 143 (7): 1099–1107.
Published: 01 April 2016
...Yinan Wan; Alexandra D. Almeida; Steffen Rulands; Naima Chalour; Leila Muresan; Yunmin Wu; Benjamin D. Simons; Jie He; William A. Harris Clonal analysis is helping us understand the dynamics of cell replacement in homeostatic adult tissues ( Simons and Clevers, 2011 ). Such an analysis, however...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2013) 140 (13): 2835–2846.
Published: 01 July 2013
... Competing interests statement The authors declare no competing financial interests. 2 5 2013 © 2013. Published by The Company of Biologists Ltd 2013 Brainbow Zebrafish Clonal analysis In vivo imaging Lineage Microscopy A deeper understanding of developmental...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2013) 140 (5): 996–1002.
Published: 01 March 2013
... correspondence ( top1214@gmail.com ) Competing interests statement The authors declare no competing financial interests. 17 12 2012 © 2013. Published by The Company of Biologists Ltd Clonal analysis Fate determination Kit Melanocyte Regeneration Stem cell Adult...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2010) 137 (23): 3931–3939.
Published: 01 December 2010
.... Organ-founding stem cell Clonal analysis Melanoblast Melanocyte Zebrafish Regeneration How the adult form arises from the embryo remains one of the greatest challenges in developmental biology. One mechanism employed by metazoans is the use of adult stem cells, which are...
Journal Articles
Journal: Development
Development (2010) 137 (4): 569–577.
Published: 15 February 2010
... understand how the HF stem cells regenerate the typical anagen organization, we conducted in vivo clonal analysis of key stages of the HF cycle in mice. Unexpectedly, we found that the pool of HF stem cells contains precursors with both multipotent and restricted contributions. This implies that the lineal...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2009) 136 (21): 3567–3574.
Published: 01 November 2009
... ideas on how cell number and type must be regulated in building complete islets of Langerhans. * Author for correspondence( pedro.herrera@unige.ch ) 27 8 2009 © 2009. 2009 Cell lineage Clonal analysis Islet Mosaic Progenitor Transgenic Mouse How organs are assembled...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2007) 134 (20): 3713–3722.
Published: 15 October 2007
... describe a general method for clonal analysis in the mouse and use it to determine the topology of clone distribution along the three limb axes. We identify a lineage restriction boundary at the limb mesenchyme dorsoventral border that is unrelated to any anatomical discontinuity, and whose lineage...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2006) 133 (4): 737–749.
Published: 15 February 2006
... embryo. This conclusion is based on a retrospective clonal analysis, using a nlaacZ reporter targeted to the α-cardiac actin gene. A rare intragenic recombination event results in a functional nlacZ sequence, giving rise to clones ofβ-galactosidase-positive cells. Periendothelial and vascular smooth...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2005) 132 (22): 5033–5042.
Published: 15 November 2005
... 58 , 787 -815. Baena-Lopez, L. A., Pastor-Pareja, J. C. and Resino, J. ( 2003 ). Wg and Egfr signalling antagonise the development of the peripodial epithelium in Drosophila wing discs. Development 130 , 6497 -6506. Baker, W. K. ( 1978 ). A clonal analysis reveals early developmental...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2005) 132 (18): 4143–4154.
Published: 15 September 2005
...., Grose, R., Revest, J. M.,Hodivala-Dilke, K. M. and Dickson, C. ( 2003 ). A crucial role for Fgfr2-IIIb signalling in epidermal development and hair follicle patterning. Development 130 , 5493 -5801. Petit, A. C., Legué, E. and Nicolas, J. F. ( 2005 ). Methods in clonal analysis and applications...
Journal Articles
Journal: Development
Development (2003) 130 (20): 4955–4962.
Published: 15 October 2003
... © 2003. 2003 Drosophila Hemocytes Clonal analysis Cell lineage Transplantation Blood GFP Lymph gland Macrophage Hematopoiesis The hemolymph of Drosophila contains hemocytes that either circulate freely through the body cavities or are sessile, being associated with various...
Journal Articles
Journal: Development
Development (2003) 130 (16): 3807–3819.
Published: 15 August 2003
... ) * Present address: Unité de Biologie Moléculaire du Développement, Institut Pasteur, 25 rue du Dr Roux, Cedex 15, 75724 Paris, France 1 5 2003 © 2003. 2003 Mouse Hox genes Hoxb1 Hoxb4 Hoxb8 Gastrulation Clonal analysis AP patterning Hox genes are key players in...
Journal Articles
Journal: Development
Development (2003) 130 (2): 321–330.
Published: 15 January 2003
... present in nascent neural crest populations and that these sublineages can be distinguished by their cell type-specific expression of receptor tyrosine kinases. * Author for correspondence (e-mail: henion.1@osu.edu ) 8 10 2002 © 2003. 2003 Neural crest Clonal analysis Lineage...
Journal Articles
Journal: Development
Development (2002) 129 (15): 3609–3619.
Published: 01 August 2002
... first time a complete clonal analysis of the mediolateral contribution of laacZ / lacZ chimeras in the mouse myotome. The description of the position of each β-gal + cell in these clones lead us to the conclusion that ML regionalisation of the precursors of the myotome can be traced back to a single...