...) are the building blocks of blood vessels, yet our understanding of EC specification remains incomplete. Zebrafish cloche/npas4l mutants have been used broadly as an avascular model, but little is known about the molecular mechanisms of action of the Npas4l transcription factor. Here, to identify its direct...

... , 1619 -1622. Liao, W., Bisgrove, B., Sawyer, H., Hug, B., Bell, B., Peters,K., Grunwald, D. and Stainier, D. ( 1997 ). The zebrafish gene cloche acts upstream of a flk-1 homologue to regulate endothelial cell differentiation. Development 124 , 381 -389. Lyons, I., Parsons, L. M., Hartley...

... for studying ocular disease in vivo. We found that the zebrafish cloche mutant, both the cloche m39 and cloche S5 alleles, which have defects in hematopoiesis and blood vessel development, also have lens cataracts. Quantitative examination of the living zebrafish lens by confocal microscopy showed significant...

... the endothelial and blood lineages may in fact function at the level of the hemangioblast. For example, the zebrafish cloche mutation disrupts the differentiation of both endothelial and blood cells. The transcription factor gene scl is expressed in both endothelial and blood lineages from an early stage and can...

... and vascular development in vertebrates. Contrary to all expectation, we find that SCL variants unable to bind DNA rescue hematopoiesis from gene-targeted SCL −/− embryonic stem cells and complement hematopoietic and vascular deficits in the zebrafish mutant cloche . Our findings establish DNA-binding...

.... and Fishman, M. C. (1993). Development 119, 31-40; Lee, R. K. K., Stainier, D. Y. R., Weinstein, B. M. and Fishman, M. C. (1994). Development 120, 3361-3366). Here we describe a zebrafish mutation, cloche , that affects both the endothelial and hematopoietic lineages at a very early stage. The endocardium...