... incubations were conducted for 2.5 days at 37°C in PBSTwH/5% DMSO/3% sheep serum. Finally, immunostained larvae ovaries were embedded in low-melting agarose 1% before proceeding to nuclear staining and clearing. Adult medaka and zebrafish ovaries were stained and cleared according to the C-ECi method...

... developed a clearing method using chemical solutions, termed ClearSee, for deep imaging of morphology and gene expression in plant tissues. ClearSee rapidly diminishes chlorophyll autofluorescence while maintaining fluorescent protein stability. By adjusting the refractive index mismatch, whole-organ...