..., in the preimplantation genetic diagnosis of human embryos. Another manifestation of this developmental plasticity is the possibility of obtaining chimaeras by combining either two embryos or embryos and pluripotent stem cells, which enables the verification of the cell pluripotency and generation of genetically modified...

... a minority of the embryonic stem cell (ESC) inoculum contributes to the adult chimaera. How incoming cells are chosen for integration or elimination remains unclear. By comparing a heterogeneous mix of undifferentiated and differentiating ESCs (serum/LIF) with more homogeneous undifferentiated culture (2i...

.../by/3.0 ), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. Blastocyst Chimaera Nanog Oct4 (Pou5f1) Primitive endoderm Sox17 A prerequisite for the viviparous development of the mouse embryo...

... ) Competing interests statement The authors declare no competing financial interests. © 2013. Published by The Company of Biologists Ltd 2013 Chimaera Developmental plasticity Regulative development Stochastic gene expression Twin One of the most intriguing observations...

...Ge Guo; Jian Yang; Jennifer Nichols; John Simon Hall; Isobel Eyres; William Mansfield; Austin Smith Mouse embryonic stem (ES) cells derived from pluripotent early epiblast contribute functionally differentiated progeny to all foetal lineages of chimaeras. By contrast, epistem cell (EpiSC) lines...

... defect of the epiblast cells and is rescued by complementation of NT blastocysts with normal ES or ICM cells. The second type of defect affects growth regulation and the shape of the embryo but does not directly impair the initial establishment of the patterning of the embryo. Only chimaeras formed...

...Marie-José Goumans; An Zwijsen; Marga A. van Rooijen; Danny Huylebroeck; Bernard A. J. Roelen; Christine L. Mummery We have analysed the function of transforming growth factor β (TGF-β) in yolk sac development in mice by generating somatic chimaeras in which the extraembryonic mesoderm, which gives...

... of Biologists 1998 Epiblast growth Clonal analysis Epiblast founder cell Intercellular bridge Visceral endoderm Coherent clonal growth Fluorescein complexon Tetramethylrhodamine dextran lysin biotin Chimaera Mouse Whilst there is little relative movement of cells during...

... to another. In contrast, a high and reproducible degree of chimaerism was obtained when the ES cells were injected at the morula stage prior to ICM formation. 25 08 1990 © 1990 by Company of Biologists 1990 embryonic stem cells chimaera mouse embryo β-galactosidase marker...

...R. S. P. Beddington; E. J. Robertson ABSTRACT Embryonic stem cells (ES) cells were injected into host blastocysts either in groups of 10–15 cells or as single cells in order to test their developmental potential in the developing embryo. The analysis of midgestation chimaeras, by electrophoretic...

...-assisted image analysis system, resulting in a clearer image of the patch shape. 19 07 1988 © 1988 by Company of Biologists 1988 chimaera clonal analysis mouse beige mutation kidney proximal tubule liver parenchyma Chimaeric animals have been used for the analysis of cell...

... on chimaeric villi in the neonate shows a mottled pattern of mosaicism which is progressively replaced by coherent sheets of cells from the crypts, and within two weeks the orderly adult clonal pattern is established. © 1988 by Company of Biologists 1988 chimaera mouse intestine crypt killus...

...K. M. Bagnall; S. J. Higgins; E. J. Sanders The somitic involvement in the formation of the vertebral column was examined using the chick-quail chimaera model. Single cervical somites from quail donor embryos were transplanted into similarly staged chick host embryos. Following further incubation...

... if similar influences are associated with embryonic fibre-type differentiation, the limb musculature of intraspecific (chick/chick) chimaeras was assessed using differential myosin-ATPase activity. The limb musculature analysed histochemically was derived from donor unsegmented thoracic somitic mesoderm...

...A. Nagy; A. Páldi; L. Dezso; L. Varga; A. Magyar ABSTRACT Parthenogenetically activated BCF1 and fertilized BALB/c embryos were aggregated to form chimaeras. The fate of the parthenogenetic component was followed in the conceptus during the second half of gestation. The results indicate an early...

... was markedly different. There were no stripes anywhere from the midthoracic region to the groin, but a straight midventral clonal boundary was observed. These findings were consistent in chimaeras of each of the three strain combinations studied. The lack of obvious ventral striping in epidermis suggests...

... of patches per unit area was consistent between the simulation and the chimaeric tissue. * To whom correspondence should be addressed 06 10 1986 © 1987 by Company of Biologists 1987 rat liver chimaera pattern formation mosaic tissues computer simulation Chimaeric...

...B. K. Waters; J. Rossant ABSTRACT Certain embryonal carcinoma (EC) cell lines can colonize the embryo following blastocyst injection or embryo aggregation, giving rise to EC-embryo chimaeras. However, such chimaeras often develop abnormally. For example, diploid P19 cells colonize the embryo...

... phagosome content. The receptor cells also degenerate at a slower rate than in the homozygotes. The nature of the interaction resulting in dilution of the retinal lesion in the heterozygous retina was analysed in a series of chimaeras consisting of rds/ + and +/+ genotypes, which also differed in colour...

... chimaeras were produced by transplantation of corresponding quail tissue in the region of somites 18–21. A morphometrical analysis of the mesonephric and gonadal area in cross sections shows that the intermediate mesoderm from somites 16 to 23 develops into the mesonephros. A partial agenesis...