... expression. In this study, we report a novel regulatory mechanism for Hh in LR asymmetry development of amphioxus embryos. Our results revealed that Hh −/− embryos abolish Cerberus ( Cer ) transcription, with bilaterally symmetric expression of Nodal , Lefty and Pitx . In consequence, Hh −/− mutants...

...Ana Teresa Tavares; Sofia Andrade; Ana Cristina Silva; José António Belo The TGF-β-related molecule Nodal plays an essential and conserved role in left-right patterning of the vertebrate embryo. Previous reports have shown that the zebrafish and mouse Cerberus-related proteins Charon and Cerberus...

...Ana Cristina Silva; Mario Filipe; Klaus-Michael Kuerner; Herbert Steinbeisser; José António Belo We analyzed the endogenous requirement for Cerberus in Xenopus head development. `Knockdown' of Cerberus function by antisense morpholino oligonucleotides did not impair head formation in the embryo...

... part of the dorsal telencephalic inducing activities may be reproduced by the anterior endoderm secreted molecule cerberus, but not by simple BMP inhibition, and requires the N-terminal region of cerberus that includes its Wnt-binding domain. Furthermore, we show that FGF action is both necessary...

... of temporal expression patterns by RT-PCR in Xenopus embryos. derrière transcripts are first detected at stage 8.5 at the same time as xnr1 and xnr4 as well as the homeobox gene mix1 . Transcription of the mesodermal marker xbra and the dorsal specific marker cerberus are detected soon...

...Eric Agius; Michael Oelgeschläger; Oliver Wessely; Caroline Kemp; E. M. De Robertis ABSTRACT In Xenopus , mesoderm induction by endoderm at the blastula stage is well documented, but the molecular nature of the endogenous inductive signals remains unknown. The carboxy-terminal fragment of Cerberus...

... of activation of FGF or activilike signal transduction pathways but may reflect the activation of different targets of Siamois. Chordin , a marker of the head and axial mesoderm, is activated by the VCC/ Siamois pathway in animal cells but not in vegetal cells whereas cerberus , a marker of the anterior...