...Junnan Fang; Dorothy A. Lerit ABSTRACT As the microtubule-organizing centers of most cells, centrosomes engineer the bipolar mitotic spindle required for error-free mitosis. Drosophila Pericentrin-like protein (PLP) directs formation of a pericentriolar material (PCM) scaffold required for PCM...

... of Biologists Ltd 2022 Summary: The multinucleated embryo of the fruit fly D. melanogaster distributes nuclei with the help of centrosome-organised microtubule asters, with ordering dynamically adapting to synchronous nuclear divisions, mitotic failures and being independent of cortical actin...

... that the centrosomal recruitment of PCMD-1 is dependent on the outer centriolar protein SAS-7. The most C-terminal part of PCMD-1 is sufficient to target it to the centrosome, and the coiled-coil domain promotes its accumulation by facilitating self-interaction. We reveal that PCMD-1 interacts with the PCM scaffold...

... have identified protease serine 50 (PRSS50) as having a crucial role in sperm development, because Prss50 -null mice presented with impaired fertility and sperm tail abnormalities. PRSS50 could also be involved in centrosome function because these mice showed a threefold increase in acephalic sperm...

... segregation and cytokinesis, and is required for cyclin B translation in mature spermatocytes. Drosophila Male meiosis Centrosome Cytokinesis Spermatogenesis Spermatogenesis in Drosophila melanogaster offers a well-suited model system in which to investigate how transcriptional...

.... This includes regulation of both the spatial distribution of mitosis and the mode of cell division. The centrosome, which is the main microtubule-organizing centre of animal cells, contributes to both of these processes. Here, we discuss the impact that centrosome-mediated control of cell division has...

... differentiated cells regulate microtubule geometry in vivo . Although the studies described above have highlighted mechanisms that are used by various cells to inactivate the MTOC activity of centrosomes, it is not clear whether centrosomes have to be inactivated to render the cell competent to generate non...

...Sharda Prasad Yadav; Neel Kamal Sharma; Chunqiao Liu; Lijin Dong; Tiansen Li; Anand Swaroop ABSTRACT Defects in cilia centrosomal genes cause pleiotropic clinical phenotypes, collectively called ciliopathies. Cilia biogenesis is initiated by the interaction of positive and negative regulators...

... potential perturbation of the process are poorly understood. Drosophila melanogaster male germline stem cells (GSCs) possess a checkpoint, termed the centrosome orientation checkpoint (COC), that monitors correct centrosome orientation with respect to the component cells of the niche to ensure asymmetric...

... Endocycle Polyploidy Multipolar Aneuploidy Centrosome Drosophila In many post-mitotic tissues, continued growth and development is achieved by a cell cycle modification known as the endocycle. In diverse tissues, including the human trophoblast lineage, endocycles alternate DNA synthesis...

...Bertrand Jauffred; Flora Llense; Bernhard Sommer; Zhimin Wang; Charlotte Martin; Yohanns Bellaiche Asymmetric cell division generates cell fate diversity during development and adult life. Recent findings have demonstrated that during stem cell divisions, the movement of centrosomes is asymmetric...

... ). Sequence determinants of a microtubule tip localization signal (MtLS) . J. Biol. Chem. 287 , 28227 – 28242 . Buttrick G. J. , Beaumont L. M. , Leitch J. , Yau C. , Hughes J. R. , Wakefield J. G. ( 2008 ). Akt regulates centrosome migration and spindle orientation...

... controlling axon formation in vivo are poorly understood. The centrosome has been implicated in multiple aspects of neuronal morphogenesis; however, its function in axon development is under debate. Conflicting results from studies of centrosome function in axonogenesis suggest that its role is context...

... between the microtubule cytoskeleton and Wnt/PCP signaling during zebrafish gastrulation. First, we assessed the location of the centrosome/microtubule organizing center (MTOC) relative to the cell nucleus and the body axes, as a marker of cell polarity. The intracellular position of MTOCs was polarized...

... ganglia. Knockdown of IoLR5 protein results in loss of the larval eyes, which normally develop in association with these ganglia. Segregation of this RNA to the first quartet cells does not occur if centrosomal localization is bypassed. We show that the specific inheritance of the RNA by the first quartet...

... number and mitotic orientation. Our findings suggest that the reduction in brain size observed in humans with mutations in CDK5RAP2 is associated with impaired centrosomal function and with changes in mitotic spindle orientation during progenitor proliferation. Fig. 1. Hertwig's anemia...

.... the neural tube), has a mutation in KIAA0568 . Similar phenotypes are seen in mice and in human syndromes with mutations in genes that encode centrosomal or intraflagella transport proteins. Such mutations lead to defects in primary cilia, sites where Hh signalling occurs. Here, we show that cells of talpid...

... to the centrosome. The overgrowth phenotype is also enhanced by a mutation in Cdc27 , which encodes a component of the anaphase-promoting complex (APC) that regulates the degradation of cyclins. No evidence for an apoptotic function of Zfrp8 was found. Based on the phenotype, genetic interactions and subcellular...

... this transport takes place. We have identified a centriole-containing centrosome close to the oocyte nucleus. Remarkably, the centrosomal components, γ-tubulin and Drosophila pericentrin-like protein also strongly accumulate at the periphery of this nucleus. MT polymerization after cold-induced disassembly...

...Jorge Bolívar; ean-René Huynh; Hernán López-Schier; Cayetano González; Daniel St Johnston; Acaimo González-Reyes ABSTRACT During early Drosophila oogenesis, one cell from a cyst of 16 germ cells is selected to become the oocyte, and accumulates oocyte-specific proteins and the centrosomes from...