... signalling at later stages. Actomyosin cortex Tissue boundaries Planar polarity Drosophila Gastrulation Convergent extension Germband extension Parasegmental boundaries Live imaging Cell tracking MS2-MCP system LRR receptor University of Cambridge http://dx.doi.org/10.13039...

... and applications to lineage tracing in live-imaging data and outline software methodologies that can be applied to various cell-tracking challenges. * These authors contributed equally to this work ‡ Author for correspondence ( [email protected] ) Competing interests The authors...

... in vivo imaging Cell tracking Drosophila melanogaster National Institutes of Health http://dx.doi.org/10.13039/100000002 R35GM118146 American Heart Association http://dx.doi.org/10.13039/100000968 18POST34080493 California Institute Of Technology https...

... are necessary to systematically analyze epithelial organization and dynamics at single-cell resolution. We developed SEGGA, an easy-to-use software for automated image segmentation, cell tracking and quantitative analysis of cell shape, polarity and behavior in epithelial tissues. SEGGA is free, open source...

... ) Competing interests The authors declare no competing financial interests. 2 1 2014 19 5 2014 © 2014. Published by The Company of Biologists Ltd 2014 Drosophila melanogaster Epithelial folding Cell shape reconstruction Live imaging Cell tracking Cell shape analysis...

... Cell tracking Caenorhabditis elegans The interpretation of complex in vivo phenotypes is a crucial and challenging step required to achieve a functional understanding of molecular networks. Advances in imaging technology offer an unprecedented opportunity for systems-level studies...

... this intriguing concept, we developed a single-cell tracking approach that enables automatic detection of cell cycle phases in live (stem) cells expressing fluorescent ubiquitylation-based cell-cycle indicator (FUCCI) probes. Using this tool, we have identified distinctive changes in lengths and fluorescence...

... supported model. Here, we combine four-dimensional imaging with custom cell tracking software and photoactivatable fluorophore labeling to determine the cellular dynamics underlying OCM in zebrafish. Although cell division contributes to growth, we find it dispensable for eye formation. OCM depends instead...