... spiralians and to experimentally test the autonomy of D quadrant specification in mollusks and annelids with PLs and/or unequal-sized quadrant founder cells. The straightforward approach of cell ablation within respective phylogenetic contexts may reveal stepwise transitions from conditional to autonomous...

..., as recipients for gene-edited PGC transfer. We used the nitroreductase/metronidazole (NTR/Mtz) system for cell ablation, in which nitroreductase produces cytotoxic alkylating agents from administered metronidazole, causing cell apoptosis. The chicken Vasa homolog ( CVH ) gene locus was used to drive...

... testis, which are able to divide to replace missing stem cells, are unable to replace themselves. Stem cell Niche Spermatogenesis Cell ablation GeneSwitch Johns Hopkins University http://dx.doi.org/10.13039/100007880 Tissue homeostasis and repair depend on adult stem cells...

...Karen Mruk; Paulina Ciepla; Patrick A. Piza; Mohammad A. Alnaqib; James K. Chen ABSTRACT Cell ablation is a powerful method for elucidating the contributions of individual cell populations to embryonic development and tissue regeneration. Targeted cell loss in whole organisms has been typically...

...Thomas Weber; Kazuhiko Namikawa; Barbara Winter; Karina Müller-Brown; Ralf Kühn; Wolfgang Wurst; Reinhard W. Köster The zebrafish is a well-established model organism in which to study in vivo mechanisms of cell communication, differentiation and function. Existing cell ablation methods are either...

... into cellular adaptations to tissue stress. Surgical excision ( Bryant, 1975 ; Haynie and Bryant, 1976 ; Katsuyama et al., 2015 ) or cell ablation induced by pro-apoptotic transgenes ( Grusche et al., 2011 ; Herrera et al., 2013 ; Smith-Bolton et al., 2009 ) has revealed cellular responses that promote...

... by conditional cell ablation in mice that Myf5 determines a distinct myogenic cell population, which also contains some Myod1-positive cells. Ablation of this lineage uncovers the presence of a second autonomous myogenic lineage, which superseded Myf5-dependent myogenic cells and expressed Myod1. By contrast...

...Sen Wu; Yuanyuan Wu; Mario R. Capecchi Olig gene expression is proposed to mark the common progenitors of motoneurons and oligodendrocytes. In an attempt to further dissect the in vivo lineage relationships between motoneurons and oligodendrocytes, we used a conditional cell-ablation approach...

... the herpes simplex 1 virus thymidine kinase (HSVTK) under control of 1.6 kb of 5’ rat secretin gene. HSVTK expression in transgenic mice is nontoxic. However, the viral enzyme renders tissues sensitive to the nucleoside analog ganciclovir, allowing conditional cell ablation. This strategy has been used...

... ). Hence the phenotype should reflect the loss of these neurons. After cell ablation, the longitudinal axons are completely lost at stage 14 ( Fig. 3d ; Table 1 ). These defects persist throughout embryo-genesis, as detected with antibodies against both FasII ( Fig. 3g ) and BP102 ( Fig. 3h ; compare...

... pioneer growth cones. Growing axons appear to contact the glial scaffold as the axon tracts are established. We have used a novel technique for targeted cell ablation to kill the interface glia and thus to test their role in the establishment of the embryonic axon tracts. We show that ablation...

... micromeres. * Present address: Department of Anatomy and Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USA 01 09 1994 © 1994 by Company of Biologists 1994 cell lineage cell ablation fate map leech embryo micromere annelid Glossiphoniid leeches...

..., Laboratory of Mammalian Genes and Development, NICHD, NIH, Bldg. 6B, room 206, Bethesda, Maryland 20892, USA 09 October 1992 © 1993 by Company of Biologists 1993 cell lineage cell commitment cell ablation transplantation Early in their development, the embryos of annelids...

.... The results of cleavage arrest and cell ablation experiments suggest that the full range of normal cell interactions are not required for this process. However, when the animal and vegetal hemispheres are separated by re-orientation of the first cleavage plane from meridional to equatorial, the ectodermal...