... the cell cycle of stem cells remain poorly understood. Here, we show that the Cdc25 homolog String (Stg) is a crucial regulator of germline stem cells (GSCs) and cyst stem cells (CySCs) in Drosophila testis. Through knockdown and overexpression experiments, we show that Stg is required for stem cell...

... of cdc2 (synonymous with cdk1 ) and of twine , the meiotic homolog of cdc25, which is required for Cdk1 activation. We found that Twine and Polo kinase levels are reduced in endos mutants, and identified Early girl (Elgi), a predicted E3 ubiquitin ligase, as a strong Endos-binding protein. In elgi mutant...

... in myt1 females ( Table 3 , cross 2). These data demonstrate that loss of Myt1activity compromises female meiosis, specifically meiosis I. Myt1 Wee1 Cdk1 Cdc25 Meiosis Mitosis Spermatogenesis Oogenesis A conserved molecular mechanism that controls entry into mitosis in eukaryotic...

... of oogenesis). Stage IV oocytes contain a store of inactive pre-MPF (Tyr15-phosphorylated Cdc2 bound to cyclin B2); the Cdc25 phosphatase that catalyzes Tyr15 dephosphorylation of Cdc2 is also present. However, the positive feedback loop that allows MPF autoamplification is not functional at this stage...

... specification through Wnt signaling makes cells susceptible to the cdc-25.1(gf) mutation, although at present we cannot discern whether this is a direct or indirect effect. It has been shown that in other systems Wnt does affect cell division through effects on Cdc25 ( Johnston and Edgar, 1998 ; Rimerman et...