... activation of M phase-promoting factor (Cdc2 kinase-Cyclin B complex, MPF) by dephosphorylation of the inhibitory Thr14/Tyr15 sites of Cdc2. However, underlying mechanisms vary. In Xenopus oocytes, deciphering the intervening chain of events has been hampered by a sensitive amplification loop involving Cdc2...

... of oogenesis). Stage IV oocytes contain a store of inactive pre-MPF (Tyr15-phosphorylated Cdc2 bound to cyclin B2); the Cdc25 phosphatase that catalyzes Tyr15 dephosphorylation of Cdc2 is also present. However, the positive feedback loop that allows MPF autoamplification is not functional at this stage...

...Mike Boxem; Dayalan G. Srinivasanxs; Sander van den Heuvel ABSTRACT We have identified six protein kinases that belong to the family of cdc2-related kinases in Caenorhabditis elegans . Results from RNA interference experiments indicate that at least one of these kinases is required for cell-cycle...

... growth we investigated the effects of blocking cell division at different times and in spatially controlled manner by inactivation of the mitotic kinase Cdc2 in developing imaginal discs. We find that cell growth continues after inactivation of Cdc2, with little effect on overall patterning...

.... These results suggest that in G2, escargot is required to maintain a high level of G2/M cdk that actively inhibits the entry into S phase. 04 01 1996 © 1996 by Company of Biologists 1996 Since Cdc2 is constitutively present throughout the cell cycle ( Edgar et al., 1994 ), its G2-specific...

...Bodo Stern; Gabriele Ried; Nigel J. Clegg; Thomas A. Grigliatti; Christian F. Lehner ABSTRACT We have identified mutations in the Drosophila cdc2 gene. The recessive lethality of these mutant alleles was rescued after P-element-mediated transformation with a genomic cdc2 fragment. Sequence analysis...