... induced in response to Nodal signalling. Finally, we investigated the molecular basis for the FGF-MAPK-dependent repression of endoderm formation. We found that FGF/ERK signalling causes phosphorylation of Casanova/Sox32, an important regulator of endoderm determination, and provide evidence...

...Morgane Poulain; Thierry Lepage Endoderm specification in zebrafish is mediated by the zygotic transcription factors Bon/Mixer, Faust/Gata5, Casanova and Sox17, whose expression is induced by Nodal signalling. Bon/Mixer and Gata5 require Casanova in order to promote endoderm formation and all three...

.... Alexander, J., Rothenberg, M., Henry, G. L. and Stainier, D. Y. ( 1999 ). casanova plays an early and essential role in endoderm formation in zebrafish. Dev. Biol . 215 , 343 -357. Aoki, T., Mathieu, J., Saint-Etienne, L., Peyriéras, N. and Rosa, F. M. ( 2002 a). Regulation of Nodal signalling...

... for mesoderm formation. In zebrafish, downstream of Nodal signals, three genes encoding transcription factors ( faust , bonnie and clyde and the recently identified gene casanova ) are required for endoderm formation and differentiation. However their positions within the pathway are not completely established...

... into the endoderm-deficient mutant casanova . Likewise, the YSL does not appear essential for the maintenance of endodermal identity during gastrulation once the Nodal pathway has been activated. Thus, our results demonstrate that the activation of Nodal signalling is sufficient to commit cells both...

... in vertebrate endoderm formation. casanova ( cas ), another zebrafish gene, is essential for endoderm formation and functions downstream of Nodal signaling and Bon, but upstream of sox17 and foxA2 ( Alexander et al., 1999 ; Alexander and Stainier, 1999 ). Thus, the framework of a molecular pathway...