... first-trimester human hearts were studied to lay groundwork for further studies of the mechanisms underlying congenital heart defects. We first sampled 49,227 cardiac nuclei from three fetuses at 8.6, 9.0, and 10.7 post-conceptional weeks (pcw) for single-nucleus RNA sequencing, enabling the distinction...

...Whitney Edwards; Olivia K. Bussey; Frank L. Conlon ABSTRACT T-box transcription factor 20 (Tbx20) plays a multifaceted role in cardiac morphogenesis and controls a broad gene regulatory network. However, the mechanism by which Tbx20 activates and represses target genes in a tissue-specific...

... / cloche Bmp signalling Zebrafish Cardiac Australian Government Australian Research Council http://dx.doi.org/10.13039/501100000923 DP170101217 DP200103642 University of Queensland http://dx.doi.org/10.13039/501100001794 University of Melbourne http...

... present an overview of the innate and adaptive immune response to heart injury. We explore the kinetics of immune cell mobilization following cardiac injury and how the different innate and adaptive immune cells interact with one another and with the damaged tissue. We draw on key findings from...

...Aryan Kaveh; Finnius A. Bruton; Magdalena E. M. Oremek; Carl S. Tucker; Jonathan M. Taylor; John J. Mullins; Adriano G. Rossi; Martin A. Denvir ABSTRACT Sustained neutrophilic inflammation is detrimental for cardiac repair and associated with adverse outcomes following myocardial infarction (MI...

... strategies to boost mammalian heart regeneration. Nevertheless, the lack of appropriate live-imaging tools for the adult zebrafish heart has limited detailed studies into the dynamics underlying cardiomyocyte proliferation. Here, we address this by developing a system in which cardiac slices of the injured...

... respecification of anterior-like endothelium into beating cardiomyocytes. Cardiac respecification was not observed in posterior-derived endothelial cells. Thus, activin/BMP gradients specify distinct mesodermal subpopulations that generate cell derivatives with unique angiogenic, hemogenic and cardiogenic...

... to understanding the process of cardiac development and the pathology associated with human congenital heart disease. Here, we show that the cardiac transcription factor CASTOR (CASZ1) directly interacts with congenital heart disease 5 protein (CHD5), which is also known as tryptophan-rich basic protein (WRB...

..., and the loss of Tbx20 in a wide variety of model systems leads to cardiac defects and eventually heart failure. Despite the crucial role of TBX20 in a range of cardiac cellular processes, the signal transduction pathways that act upstream of Tbx20 remain unknown. Here, we have identified and characterized...

...Cammon B. Arrington; H. Joseph Yost One of the first steps in zebrafish heart and gut organogenesis is the migration of bilateral primordia to the midline to form cardiac and gut tubes. The mechanisms that regulate this process are poorly understood. Here we show that the proteoglycan syndecan 2...

...Yvette G. Langdon; Sarah C. Goetz; Anna E. Berg; Jackie Thomas Swanik; Frank L. Conlon The isolation and culturing of cardiac progenitor cells has demonstrated that growth factor signaling is required to maintain cardiac cell survival and proliferation. In this study, we demonstrate in Xenopus...

... of the embryonic cardiac cell cycle, with depletion of TBX5 leading to cardiac cell cycle arrest in late G 1 - or early S-phase. Blocking cell cycle progression by TBX5 depletion leads to a decrease in cardiac cell number, an alteration in the timing of the cardiac differentiation program, defects in cardiac...

... by overall gross morphology, molecular marker analysis and cardiac physiology, implying that the two genes are required for and have non-redundant functions in early heart development. In addition, we demonstrate that although co-expressed, Tbx5 and Tbx20 are not dependent on the expression of one another...

...Ivan P. G. Moskowitz; Anne Pizard; Vickas V. Patel; Benoit G. Bruneau; Jae B. Kim; Sabina Kupershmidt; Dan Roden; Charles I. Berul; Christine E. Seidman; Jonathan G. Seidman We report a critical role for the T-box transcription factor Tbx5 in development and maturation of the cardiac conduction...