... will further aid attempts to generate endocardial tissue and model cardiac development in vitro . All zebrafish strains were maintained and animal work performed in accordance with the guidelines of the animal ethics committee at The University of Queensland and The University of Melbourne...

... present an overview of the innate and adaptive immune response to heart injury. We explore the kinetics of immune cell mobilization following cardiac injury and how the different innate and adaptive immune cells interact with one another and with the damaged tissue. We draw on key findings from...

...Aryan Kaveh; Finnius A. Bruton; Magdalena E. M. Oremek; Carl S. Tucker; Jonathan M. Taylor; John J. Mullins; Adriano G. Rossi; Martin A. Denvir ABSTRACT Sustained neutrophilic inflammation is detrimental for cardiac repair and associated with adverse outcomes following myocardial infarction (MI...

... strategies to boost mammalian heart regeneration. Nevertheless, the lack of appropriate live-imaging tools for the adult zebrafish heart has limited detailed studies into the dynamics underlying cardiomyocyte proliferation. Here, we address this by developing a system in which cardiac slices of the injured...

... respecification of anterior-like endothelium into beating cardiomyocytes. Cardiac respecification was not observed in posterior-derived endothelial cells. Thus, activin/BMP gradients specify distinct mesodermal subpopulations that generate cell derivatives with unique angiogenic, hemogenic and cardiogenic...

... to understanding the process of cardiac development and the pathology associated with human congenital heart disease. Here, we show that the cardiac transcription factor CASTOR (CASZ1) directly interacts with congenital heart disease 5 protein (CHD5), which is also known as tryptophan-rich basic protein (WRB...

..., and the loss of Tbx20 in a wide variety of model systems leads to cardiac defects and eventually heart failure. Despite the crucial role of TBX20 in a range of cardiac cellular processes, the signal transduction pathways that act upstream of Tbx20 remain unknown. Here, we have identified and characterized...

...Cammon B. Arrington; H. Joseph Yost One of the first steps in zebrafish heart and gut organogenesis is the migration of bilateral primordia to the midline to form cardiac and gut tubes. The mechanisms that regulate this process are poorly understood. Here we show that the proteoglycan syndecan 2...

...Yvette G. Langdon; Sarah C. Goetz; Anna E. Berg; Jackie Thomas Swanik; Frank L. Conlon The isolation and culturing of cardiac progenitor cells has demonstrated that growth factor signaling is required to maintain cardiac cell survival and proliferation. In this study, we demonstrate in Xenopus...

... of the embryonic cardiac cell cycle, with depletion of TBX5 leading to cardiac cell cycle arrest in late G 1 - or early S-phase. Blocking cell cycle progression by TBX5 depletion leads to a decrease in cardiac cell number, an alteration in the timing of the cardiac differentiation program, defects in cardiac...

... by overall gross morphology, molecular marker analysis and cardiac physiology, implying that the two genes are required for and have non-redundant functions in early heart development. In addition, we demonstrate that although co-expressed, Tbx5 and Tbx20 are not dependent on the expression of one another...

...Ivan P. G. Moskowitz; Anne Pizard; Vickas V. Patel; Benoit G. Bruneau; Jae B. Kim; Sabina Kupershmidt; Dan Roden; Charles I. Berul; Christine E. Seidman; Jonathan G. Seidman We report a critical role for the T-box transcription factor Tbx5 in development and maturation of the cardiac conduction...