... observational methods, whereas perturbational approaches have lacked precision. CRISPR-Cas9 has begun to transform the study of gene regulation, allowing for precise manipulation of genomic sequences, epigenetic functionalization and gene expression. CRISPR-Cas9 technology has already led to the discovery...

... the versatility of our engineered RIA-CRISPR viruses. This single-plasmid approach could also be incorporated in other virus families, including adeno- and lenti-viruses for application in other species. Moreover, we have expanded the application of the CRISPR-Cas9 system to investigations involving live imaging...

... and post-migrating NM cells. FMRP downregulation induced by CRISPR/Cas9 and shRNA techniques resulted in perturbed axonal pathfinding, delay in midline crossing, excess branching of neurites, and axonal targeting errors during the period of circuit development. Together, these results provide the first...

... differentiation are still poorly understood. Using CRISPR-Cas9 we re-examine the function of Distal-less (Dll) as an activator or repressor of eyespots, a topic that remains controversial. We show that the phenotypic outcome of CRISPR mutations depends upon which specific exon is targeted. In Bicyclus anynana...

... that transient dual-SMAD inhibition, together with retinoic acid (dSMADi-RA), provides rapid and reproducible induction of human spinal cord progenitors from neuromesodermal progenitor-like cells. Using CRISPR-Cas9 to engineer human embryonic stem cells with a GFP-reporter for neuromesodermal progenitor...

...Alexa Burger; Helen Lindsay; Anastasia Felker; Christopher Hess; Carolin Anders; Elena Chiavacci; Jonas Zaugg; Lukas M. Weber; Raul Catena; Martin Jinek; Mark D. Robinson; Christian Mosimann CRISPR-Cas9 enables efficient sequence-specific mutagenesis for creating somatic or germline mutants...