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Keywords: CRISPR
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Journal Articles
Journal: Development
Development (2021) 148 (19): dev199536.
Published: 13 October 2021
... of the majority of these maternally expressed factors is not fully understood. By exploiting the biallelic editing ability of CRISPR-Cas9, we identify and characterize maternal-effect genes in a single generation, using a maternal crispant technique. We validated the ability to generate biallelic mutations...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2021) 148 (19): dev199994.
Published: 04 October 2021
... requirements during zebrafish somitogenesis . Dev. Cell , 8 , 575 - 586 . 10.1016/j.devcel.2005.01.016 Kimura , Y. , Hisano , Y. , Kawahara , A. and Higashijima , S . ( 2014 ). Efficient generation of knock-in transgenic zebrafish carrying reporter/driver genes by CRISPR/Cas9...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2021) 148 (6): dev196964.
Published: 21 March 2021
... developmental stage, we identified 81 zinc finger protein (ZFP) genes that are preferentially expressed during endocrine specification and β-cell maturation. Third, we used the GCN to select three ZFPs for further analysis by CRISPR mutagenesis of mice. Zfp800 null mice exhibited early postnatal lethality...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2021) 148 (2): dev195586.
Published: 20 January 2021
... License ( https://creativecommons.org/licenses/by/4.0 ), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. Summary: In utero CRISPR AAV9-PHP.eB provides a powerful platform to efficiently manipulate gene expression...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2020) 147 (9): dev184481.
Published: 12 May 2020
... in question, which limit their application. Here, we introduce a new method that completely avoids the need for prior genetic engineering, by identifying endogenous CRISPR/Cas9 target arrays suitable for lineage analysis. In both mouse and zebrafish, we identify the highest quality compact arrays as judged...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2019) 146 (1): dev170969.
Published: 04 January 2019
... is sometimes difficult to achieve. Here, we present Valcyrie, a new method that replaces the conventional Cre or Flp recombinase-mediated excision of a marker cassette by CRISPR-mediated excision. A major advantage of this approach is that CRISPR efficiency can be tuned in a predictable fashion by manipulating...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2018) 145 (4): dev160333.
Published: 23 February 2018
...Ruth M. Williams; Upeka Senanayake; Mara Artibani; Gunes Taylor; Daniel Wells; Ahmed Ashour Ahmed; Tatjana Sauka-Spengler ABSTRACT CRISPR/Cas9 genome engineering has revolutionised all aspects of biological research, with epigenome engineering transforming gene regulation studies. Here, we present...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2018) 145 (2): dev156844.
Published: 29 January 2018
... , L. , Ran , F. A. , Cox , D. , Lin , S. , Barretto , R. , Habib , N. , Hsu , P. D. , Wu , X. , Jiang , W. , Marraffini , L. A. , et al. ( 2013 ). Multiplex genome engineering using CRISPR/Cas systems . Science   339 , 819 - 823 . 10.1126/science.1231143...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2017) 144 (19): 3562–3577.
Published: 01 October 2017
.... Collectively, these findings demonstrate that miR-322 is an unconventional inhibitor of a central signaling pathway in chondrocytes that regulates transient and permanent cartilage homeostasis. MEK Cartilage MAPK miR-322 CRISPR Stabilization Summary: miR-322 is identified...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2017) 144 (2): 345–355.
Published: 15 January 2017
... be used to resolve multiple roles of Nodal signaling at different stages of embryonic development. Finally, we show how inducible gene editing can be achieved by combining the TAEL and CRISPR/Cas9 systems. This toolkit should be a broadly useful resource for the fish community. * Present address...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2017) 144 (1): 3–7.
Published: 01 January 2017
... the CRISPR-Cas9 genome editing system and discuss its potential applications and limitations in human pre-implantation embryos, and the ethical considerations thereof. * Author for correspondence ( fredrik.lanner@ki.se ) Competing interests The authors declare no competing or financial interests...
Journal Articles
Journal: Development
Development (2016) 143 (17): 3216–3222.
Published: 01 September 2016
... brain. Electroporation of donor DNA with the CRISPR/Cas9 system vectors successfully leads to knock-in of the donor sequence, such as EGFP , to the target site via the homology-directed repair mechanism. We developed a targeting vector system optimized to prevent anomalous leaky expression of the donor...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2016) 143 (13): 2344–2355.
Published: 01 July 2016
.... Using CRISPR/Cas9-mediated genome editing, we generated and analyzed developmental defects in mice carrying additional Golgb1 loss-of-function mutations, which supported a crucial requirement for Golgb1 in palate development. Through maxillary explant culture assays, we demonstrate that the Golgb1...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2016) 143 (1): 15–23.
Published: 01 January 2016
... with silencing of exogenous OSKM, and upregulation of endogenous OCT4 . A CRISPR/Cas9-mediated GFP knock-in visualized the temporal regulation of endogenous OCT4 in cells converting from iRSC to iPSC state. OCT4 activation coincident with silencing of OSKM occurred prior to entering MET. Notably, OCT4...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2015) 142 (23): 4180–4187.
Published: 01 December 2015
... and evolution of vertebrate physiology, morphology and development. Despite its key phylogenetic position, studies of lamprey have been limited by their complex life history, which makes traditional genetic approaches impossible. The CRISPR/Cas9 system is a bacterial defense mechanism that was recently adapted...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2015) 142 (11): 2069–2079.
Published: 01 June 2015
... , generated using CRISPR-Cas9 technology, and embryos without Lhx1 function specifically in the anterior mesendoderm displayed head defects that partially phenocopied the truncation defects of Lhx1- null mutants. Therefore, disruption of Lhx1 -dependent movement of the anterior mesendoderm cells and failure...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2014) 141 (10): 2165–2171.
Published: 15 May 2014
... of this process has been limited. Here, we report that the CRISPR-Cas9 RNA-guided nuclease system can efficiently create mutations at targeted sites within the axolotl genome. We identify individual animals treated with RNA-guided nucleases that have mutation frequencies close to 100% at targeted sites. We employ...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2014) 141 (3): 707–714.
Published: 01 February 2014
... the CRISPR/Cas system ( Cradick et al., 2013 ; Fu et al., 2013 ; Hsu et al., 2013 ), our data suggest that the gRNA/Cas9-induced off-target mutation rate is very low in X. tropicalis embryos, consistent with data obtained with mouse embryos ( Yang et al., 2013 ). Future studies using whole-genome...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2014) 141 (1): 219–223.
Published: 01 January 2014
... regulatory networks in these processes and thereby identify nodes where signal input will direct desired cell fate decisions in vitro or in vivo . To deconvolute these networks, we established a method to influence the differentiation state of hPSCs with a CRISPR-associated catalytically inactive dCas9 fused...
Includes: Supplementary data