... embryonic stem cell differentiation system to determine if and how RUNX1 dosage affects hemogenic endothelium differentiation. The use of inducible Runx1 expression combined with alterations in the expression of the RUNX1 co-factor CBFβ allowed us to evaluate a wide range of RUNX1 levels. We demonstrate...

...Hiroshi Kagoshima; Rachael Nimmo; Nicole Saad; Junko Tanaka; Yoshihiro Miwa; Shohei Mitani; Yuji Kohara; Alison Woollard In this report, we investigate the C. elegans CBFβ homologue,BRO-1. bro-1 mutants have a similar male-specific sensory ray loss phenotype to rnt-1 (the C. elegans homologue...

... transcriptional repressor. The dominant-negative model posits that AML1-ETO interferes with the function of endogenous RD proteins such as AML1 by competing for a common co-factor. The best characterized co-factor for the RD family of transcription factors is CBFβ, which has two Drosophila homologs, Bro...

... of proteins and the β-subunit is encoded by the CBFβ gene. In Drosophila, two genes encoding α-subunits, runt and lozenge , and two genes encoding β-subunits, Big brother and Brother, have been previously identified. Here, a sensitized genetic screen was used to isolate mutant alleles of the Big brother gene...