... mammary organoids (EqMaOs) from cryopreserved mammary tissue, in which mammary tissue fragments were isolated and embedded into a 3D matrix to produce EqMaOs. We evaluated viability, proliferation and budding capacity of EqMaOs at different time points during culture, showing that although the number...

...Kaz Kawamura; Seigo Kitamura; Satoko Sekida; Masayuki Tsuda; Takeshi Sunanaga Zooids of the asexual strain of Polyandrocarpa misakiensis have a lifespan of 4-5 months; before dying, they produce many buds, enabling continuation of the strain. This study was designed to investigate the nature...

... Chordates Tunicates Asexual development Budding Embryogenesis Evolution Cephalochordates (including Amphioxus ; see Glossary, Box 1 ), tunicates (or urochordates, see Glossary, Box 1 ) and vertebrates constitute the chordate phylum, which is characterized by a tadpole-like body plan...

..., suggesting that vasa might play a homeostatic role in asexual development. B. schlosseri is a colonial ascidian with three co-existing generations arranged spatially into a star-shaped group called a system,containing both adults (zooids) and buds, all connected by a common vasculature ( Fig. 1...

... Hydra , we identified a receptor tyrosine kinase ( kringelchen ) with high similarity to FGFR tyrosine kinases. The gene is dynamically upregulated during budding, the asexual propagation of Hydra . Activation occurs in body regions, in which the intrinsic positional value changes. During tissue...

...Jun Matsumoto; Chiaki Nakamoto; Shigeki Fujiwara; Toshitsugu Yubisui; Kazuo Kawamura We have isolated two Ca 2+ -dependent, galactose-binding polypeptides from the budding tunicate, Polyandrocarpa misakiensis . Based on their partial amino acid sequences, full-length cDNAs were cloned. One of them...

... is a marker for synchronously dividing nematoblasts while prdl-a displays an expression restricted to the the nerve cell lineage of the head region. During budding and apical regeneration, an early and transient prdl-a expression was observed in endodermal cells of the stump at a time when the head organizer...

...Kazuo Kawamura; Kenji Hara; Shigeki Fujiwara ABSTRACT We have extracted retinoids from the budding tunicate Polyandrocarpa misakiensis and, using HPLC, identified some major peaks as cis- retinal, all- trans- retinal and all- trans- retinoic acid, of which cis- retinal was most abundant (∼2 M...

...Kazuo Kawamura; Shigeki Fujiwara; Yasuo M. Sugino ABSTRACT We have examined immunocytochemically the expression, localization and in vivo function of a calciumdependent and galactose-binding 14 ×10 3 M r lectin purified from the budding tunicate, Polyandrocarpa misakiensis . Lectin granules first...

...Jun Takano; Tsutomu Sugiyama ABSTRACT Effect of food was examined on the budding rate and the developmental gradients of a mutant hydra strain L4. This mutant strain has very high levels of head-inhibition potential gradient along its body axis ( Takano & Sugiyama, 1983 ). It also has a reduced...