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Keywords: Bimolecular fluorescence complementation (BiFC)
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Journal Articles

Visualization of the Drosophila dKeap1-CncC interaction on chromatin illumines cooperative, xenobiotic-specific gene activation

Huai Deng, Tom K. Kerppola
Journal: Development
Development (2014) 141 (16): 3277–3288.
DOI: https://doi.org/10.1242/dev.110528
Published: 15 August 2014
... cells in Drosophila . This method is based on bimolecular fluorescence complementation (BiFC) analysis of protein interactions on polytene chromosomes. Drosophila Keap1 (dKeap1)-CncC complexes localized to the nucleus and bound chromatin loci that were not bound preferentially by dKeap1 or CncC when...
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Includes: Supplementary data
Journal Articles

Rab5-mediated endocytosis of activin is not required for gene activation or long-range signalling in Xenopus

Anja I. Hagemann, Xin Xu, Oliver Nentwich, Marko Hyvonen, James C. Smith
Journal: Development
Development (2009) 136 (16): 2803–2813.
DOI: https://doi.org/10.1242/dev.034124
Published: 15 August 2009
... ligand distribution and Smad2/Smad4 bimolecular fluorescence complementation (BiFC) to analyse, in a quantitative manner, the cellular response to induction. Our results indicate that labelled activin travels exclusively through the extracellular space and that its range is influenced by numbers of type...
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Includes: Multimedia, Supplementary data