...Xiaorei Sai; Yayoi Ikawa; Hiromi Nishimura; Katsutoshi Mizuno; Eriko Kajikawa; Takanobu A. Katoh; Toshiya Kimura; Hidetaka Shiratori; Katsuyoshi Takaoka; Hiroshi Hamada; Katsura Minegishi ABSTRACT For left-right symmetry breaking in the mouse embryo, the basal body must become positioned...

... of the ciliary basal body (BB). Using the monociliated epithelium of the embryonic zebrafish floor-plate, we investigated the dynamics and mechanisms of BB polarization by live imaging. BBs were highly motile, making back-and-forth movements along the antero-posterior (AP) axis and contacting both the anterior...

... in most Lis-1 spermatocytes; centrosomes that do break cortical associations fail to attach to the nucleus. In Lis-1 spermatids, we observed loss of attachments between the nucleus, basal body and mitochondria. The localization pattern of LIS-1 protein throughout Drosophila spermatogenesis mirrors...

.... Moreover, basal bodies are mispositioned along both the apicobasal and planar polarity axes of mutant hair cells, and hair bundle orientation was uncoupled from the basal body position. We show that a non-ciliary function of Kif3a regulates localized cortical activity of p21-activated kinases (PAK), which...

... that C2cd3 is an essential regulator of intracellular transduction of the Hedgehog signal. Furthering a link between Hedgehog signaling and cilia function, we find that cilia formation and proteolytic processing of Gli3 are disrupted in C2cd3 mutants. Finally, we observe C2cd3 protein at the basal body...

... mice that produce bioactive, fluorescently labeled Shh from the endogenous locus. We show that Shh ligand concentrates in close association with the apically positioned basal body of neural target cells, forming a dynamic, punctate gradient in the ventral neural tube. Both ligand lipidation and target...

... contributed equally to this work † Present address: Instituto de Investigaciones Biomédicas, Consejo Superior de Investigaciones Científicas, Arturo Duperier 4, 28029 Madrid, Spain 7 6 2007 © 2007. 2007 Evc Ihh Hedgehog signalling Chondrocyte Basal body Gli3 processing...

...Jeanette Vierkotten; Renate Dildrop; Thomas Peters; Baolin Wang; Ulrich Rüther In this study we show in mice that Ftm (Rpgrip1l) is located at the ciliary basal body. Our data reveal that Ftm is necessary for developmental processes such as the establishment of left-right asymmetry and patterning...

... of the cortical disorders and their development during division, using various specific antibodies labelling either one of the cortical cytoskeleton components, as was previously done for analysis of cortical pattern formation in the wild type. Several abnormalities in basal body properties were consistently...

...Norman E. Williams; Jerry E. Honts; Janina Kaczanowska Differentiated regions within the membrane skeleton are described around basal bodies in the ciliary rows of Tetrahymena . These domains, approximately 1μm in diameter, are characterized by a relatively dense ultra-structure, the presence...

...Noël De Terra ABSTRACT In the ciliate Stentor , many thousands of basal bodies assemble on the ventral cell surface to form a new oral apparatus during cell division, regeneration and reorganization (oral replacement during interphase). During interphase, oral development is normally inhibited...

...Noël De Terra ABSTRACT My previous work has shown that disconnecting the oral apparatus of Stentor into two parts induces mass assembly of basal bodies on the ventral cell surface and thus initiates oral development. This operation severs the extensive microtubule tracts joining the oral...