... specifically in the trigeminal nerve and in spinal motor and sensory neurons in a Brain-derived neurotrophin factor (BDNF)-dependent manner. Knockdown of Spry3 expression causes an excess of axonal branching in spinal cord motoneurons in vivo. Furthermore, Spry3 inhibits the ability of BDNF to induce filopodia...

... of a developmental switch in the intracellular signalling network required for an identical cellular response to the same extracellular signal in the same cell type. We show that although NF-κB signalling is required for BDNF-promoted neurite growth from both foetal and postnatal mouse sensory neurons...

... to show that the neural crest does not supply cells to taste buds, either embryonically or postnatally, thus ruling out a mesenchymal contribution to taste buds. Finally, using Bdnf null mice, which lose neurons that innervate taste buds, we demonstrate that Shh-expressing taste bud progenitors...

... in embryonic precursor cell proliferation both in culture and in vivo. Inhibition of TrkB/C also caused a delay in the generation of neurons, but not astrocytes, and ultimately perturbed the postnatal localization of cortical neurons in vivo. Conversely, overexpression of BDNF in cortical precursors in vivo...

..., activation of the P2X receptor signaling pathway by ATPγS or α,βMeATP inhibited BDNF-induced neurite outgrowth and branching. These findings indicate that P2X receptor signaling provides a mechanism for inhibiting neurotrophin support of SGN neurites when synaptic reorganization is occurring in the cochlea...

... properties. Preadsorption of CM Cerebellum with antisera against candidate cytokines showed that TGFβ2 and BDNF could account for the major part of the anti-proliferative and pro-differentiating activities, an interpretation strengthened by studies involving treatment with purified TGFβ2 and BDNF...

... inhibitor. Fig. 6A shows that ALLN did not impair the survival of P0 nodose neurons grown with BDNF. At the same dose, however, ALLN caused marked and highly significant decreases in the length and branching of neurites( Fig. 6,C ). Likewise, Sholl analysis revealed marked decreases in neurite branching...

... of the mature cochlea is currently unknown. Here, we have analysed the consequences of a lack of the TrkB receptor and its ligand, the neurotrophin brain-derived neurotrophic factor (Bdnf), in the late postnatal or adult cochlea using mouse mutants. During early postnatal development, mutant animals show a lack...

... ; Fariñas et al.,1996 ) and BDNF ( Ernfors et al.,1994a ; Jones et al.,1994 ), did not reveal any developmental abnormality in the LC. Provided that both GDNF and NT3 have been reported to prevent the degeneration of adult LC NA neurons in lesion models( Arenas and Persson, 1994 ; Arenas et al., 1995...

.... Neurotrophin specificity has been attributed to the selective expression of the Trk tyrosine kinase receptors in different neuronal subpopulations. However, despite overlapping expression of TrkB and TrkC in many sensory ganglia, brain-derived neurotrophic factor (BDNF) and neurotrophin 3 (NT3) null mutant...

..., including migration, neural differentiation and formation and refinement of connections. The mechanisms regulating spontaneous activity are not known. By using transgenic embryos that overexpress BDNF under the control of the nestin promoter, we show here that BDNF controls the emergence and robustness...

... vision. We now show that infusions of NT-4/5 and NGF (nerve growth factor) into visual cortex at the onset and the peak of the critical period accelerated LGN neuron growth. BDNF (brain-derived neurotrophic factor) was ineffective. The effects of neurotrophin on LGN development were clearly dissociated...

... these zones. Here we show that migration of cerebellar granule cells out of their proliferative zone, the external granule cell layer (EGL), is impaired in Bdnf –/– mice. The reason for impaired migration is that BDNF directly and acutely stimulates granule cell migration. Purified Bdnf –/– granule cells show...

...-derived neurotrophic factor (BDNF), a TrkB ligand. As in NT3 and TrkC null mice, the proprioception system of these mutants failed to assemble. However, sensory fiber projections in the embryonic spinal cord suggest chemotropic effects of BDNF in vivo. In the dorsal root ganglia, the developmental dynamic...

...Yasushi Enokido; Sean Wyatt; Alun M. Davies ABSTRACT Previous studies have shown that most neurons in cultures established during the early stages of neurogenesis in the embryonic mouse trigeminal ganglion are supported by BDNF whereas most neurons cultured from older ganglia survive with NGF...

... (NGF) acting via its p75 receptor (Frade, J. M., RodrÍguez-Tébar, A. and Barde, Y.-A. (1996) Nature 383, 166-168). Here, we show that the application of brain-derived neurotrophic factor (BDNF) to chick embryos in ovo prevented retinal cell death in the early period, whereas exogenously applied NGF...

... study, we examined the developing vestibulo-cochlear system to determine the time course of neurotrophin dependence and to determine whether competition occurred among developing cochlear or vestibular neurons for available amounts of either brain-derived neurotrophic factor (BDNF) or neurotrophin-4/5...

... preferences are due to a nontrophic effect of NGF. Unlike DRG cultured in NGF, those cultured in the presence of either NT-3 or BDNF often exhibited neurite growth preferences for the spinal cord cryosections; the effect was strongest in cultures containing NT-3. In addition, while a mixture of insulin...

...Anna Buj-Bello; Luzia G. P. Pinon; Alun M. Davies ABSTRACT Recent work has shown that the survival of the nerve growth factor (NGF)-dependent trigeminal ganglion neurons of the mouse embryo is promoted by brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) during the early stages...