... embryos for normal AP patterning is less well understood. To investigate this process in an invertebrate deuterostome, we defined Axin function in early sea urchin embryos. Axin is ubiquitously expressed at relatively high levels in early embryos and functional analysis revealed that Axin suppresses...

...Daniel B. Lybrand; Misha Naiman; Jessie May Laumann; Mitzi Boardman; Samuel Petshow; Kevin Hansen; Gregory Scott; Marcel Wehrli ABSTRACT The central regulator of the Wnt/β-catenin pathway is the Axin/APC/GSK3β destruction complex (DC), which, under unstimulated conditions, targets cytoplasmic β...

...Yetis Gultekin; Hermann Steller ABSTRACT Self-renewal of intestinal stem cells is controlled by Wingless/Wnt-β catenin signaling in both Drosophila and mammals. As Axin is a rate-limiting factor in Wingless signaling, its regulation is essential. Iduna is an evolutionarily conserved ubiquitin E3...

...) have become lead therapeutic candidates for Wnt-driven cancers, following the recent discovery that Tnks targets Axin, a negative regulator of Wnt signaling, for proteolysis. Initial reports indicated that Tnks is important for Wnt pathway activation in cultured human cell lines. However...

... test several hypotheses related to the mechanism of action of APC2, revealing that APC2 functions at the embryonic cortex with several protein partners, including Axin, to promote mitotic fidelity. Our in vivo data demonstrate that APC2 protects genome stability by modulating mitotic fidelity through...

... a threshold for the amount of this form of Armadillo and therefore for Wnt signalling. In the current model of Wnt signalling, a complex assembled around Axin and Apc allows GSK3 (Shaggy) to phosphorylate Armadillo and target it for degradation. However, genetic experiments suggest that the loss of function...

...) initiates Wnt/β-catenin signaling. Here we show that both Fz and Dvl functions are critical for Wnt-induced Lrp6 phosphorylation through Fz-Lrp6 interaction. We also show that axin, a key scaffolding protein in the Wnt pathway, is required for Lrp6 phosphorylation via its ability to recruit Gsk3...

... that promotes forebrain development and inhibits Wnt signaling by activating the transcription of secreted Wnt antagonists. * Author for correspondence (e-mail: [email protected] ) 12 6 2006 © 2006. 2006 Axin Forebrain Lhx5 LIM-homeobox domain factor masterblind Secreted...

... sections of the β-gal stained metopic sutures, counterstained with nuclear Fast Red. Scale bars: 3 mm in A,H; 5 mm in B-D; 1 mm in E,F; 2 mm in G; 100 μm in I-L. The involvement of Axin family genes in the regulation of β-catenin stability ( Behrens et al.,1998 ; Hsu et al.,2001 ; Zeng et al...

... for subdivision of midline neural tissue into hypothalamic and floorplate domains are uncertain, and in this study, we have explored the role of the Wnt/Axin/β-catenin pathway in this process. This pathway has been implicated in anteroposterior regionalisation of the dorsal neural tube but its role in patterning...

... tissue is transformed not only into head cuticle but also into a variety of inappropriate structures. * Author for correspondence (e-mail: [email protected] ) 15 8 2002 ©2002. 2002 Drosophila Wingless Axin Eyes absent Sine oculis Dachshund Eye Transdetermination...

... that mutations in axin prevent photoreceptor differentiation and lead to tissue overgrowth and that both these effects are due to ectopic activation of the Wingless pathway. In addition, ectopic Wingless signaling causes posterior cells to take on an anterior identity, reorienting the direction of morphogenetic...

...Karl Willert; Catriona Y. Logan; Anup Arora; Matt Fish; Roel Nusse ABSTRACT The vertebrate Axin protein, the product of the mouse fused gene, binds to β-catenin to inhibit Wnt signaling. We have identified a homolog of Axin in Drosophila, Daxin . Using double-stranded RNA interference, we generated...