.../Notch activity, indicating that lag-1 engages in direct positive autoregulation. Deletion of the HOT region from endogenous lag-1 reduced LAG-1 levels and abrogated positive autoregulation, but did not cause hallmark cell fate transformations associated with loss of lin-12 / Notch or lag-1 activity...

... that such autoregulation serves to maintain the differentiated state of a cell. To experimentally test this assumption, we used CRISPR/Cas9-mediated genome engineering to delete a transcriptional autoregulatory, cis -acting motif in the che-1 zinc-finger transcription factor locus, a terminal selector required to specify...

[email protected] ) 31 7 2008 © 2008. 2008 Hox Ultrabithorax Polycomb Autoregulation engrailed The Hox genes specify the anteroposterior (A/P) axis of bilaterians( Lewis, 1978 ; Duboule, 2007 ). They are expressed in defined domains along this axis and their mis-expression...

... Pax6 in a normal spatial pattern, with levels up to three times higher than wild type. By crossing PAX77 mice with a new YAC transgenic line that reports Pax6 expression (DTy54), we showed that increased expression is limited by negative autoregulation. Increased expression reduces proliferation...

... ganglia of Brn3a +/+ and Brn3a +/- mice are similar, demonstrating that autoregulation can compensate for the loss of one allele by increasing transcription of the remaining gene copy. Conversely, transgenic overexpression of Brn3a in the trigeminal ganglion suppresses the expression of the endogenous...

... activation and maintenance of α-enhancer driven expression in vivo. It is tempting to speculate that such an autoregulatory mechanism might underlie the particular sensitivity of the iris to the Pax6 dose, possibly via amplifying small decreases in Pax6 levels. Positive autoregulation via direct interaction...

... when Cctra autoregulation is prevented and instead splice variants with truncated open reading frames are produced. We propose that this repression is achieved by the Y-linked male-determining factor ( M ). *Author for correspondence (e-mail: [email protected] ) 30 4 2002 ©...

...-specific allele, da lyh , we show that da expression is not constitutive, but is dynamically regulated. This transcriptional regulation includes somatic ovary-specific activation, autoregulation and negative regulation. Unexpectedly, the diverse functions of da may require that expression levels be tightly...

... for correspondence (e-mail: [email protected] ) 27 6 2001 © 2001. 2001 Ascidian T-box gene As-T2 Function Minimal promoter T protein-binding motif Autoregulation Muscle-specific gene expression The T-box genes encode a family of transcription factors that share...

... provide the first in vivo evidence that gata1 gene expression undergoes positive autoregulation. *Author for correspondence (e-mail: [email protected] ) 19 3 2001 © 2001. 2001 Autoregulation First intron GATA1 GFP reporter gene Hematopoiesis Transactivation Transgenic...

... Oogenesis Autoregulation Translation Drosophila Axis determination in the Drosophila embryo depends upon mRNA localization pathways that operate during oogenesis (reviewed by St. Johnson and Nusslein-Volhard, 1992). The posterior axis is established by the posterior organizing factor Oskar...

... activities of the head-gap genes buttonhead and empty spiracles and the pair-rule gene even skipped ; it therefore integrates inputs from both the head and trunk segmentation systems, which were previously considered as being essentially independent. After gastrulation, positive autoregulation of col...

.../lacZ ) is either abolished or significantly reduced in all tissues at E10.5-E12.5. Thus, the observed autoregulation of the Drosophila Deformed gene is conserved in a mouse homolog in vivo, and is reflected in a widespread requirement for positive feedback to maintain Hoxa4 expression. We also identify...

... by autoregulation and depends on virilizer (vir) . This gene is required in somatic cells for the female-specific splicing of Sxl primary transcripts and for an unknown vital process in both sexes. In the soma, clones of XX cells lacking Sxl or vir are sexually transformed and form male structures; in the germline...

... Xenopus cement gland otx2 hormone-inducible protein autoregulation The Xenopus cement gland is a mucus-secreting organ that forms from the anterior-most ectoderm at the dorsoventral border and corresponds to the chin primordium of mammals. The cement gland primordium becomes apparent...

... in Drosophila . Development 112 , 755 – 761 . 10.1242/dev.112.3.755 Bienz , M. and Tremml , G. ( 1988 ). Domain of Ultrabithorax expression in Drosophila visceral mesoderm from autoregulation and exclusion . Nature 333 , 576 – 578 . 10.1038/333576a0 Boulay , J. L...

... hunchback segmentation gap gene tailless huckebein autoregulation transcription repression Segmentation in the Drosophila embryo is a hierarchical process that gradually divides the embryo into increasingly finer subsets of cells ( Akam, 1987 ; Ingham, 1988 ). Three distinct maternal...

... autoregulation of the endogenous teashirt gene and the presence of Sex combs reduced protein in the labium explain this homeosis. Patterns in the maxillary and a more anterior head segment are partly replaced with trunk ones. Additional Teashirt protein has no effect on the identity of the trunk segments where...

... in the midgut depends on the dpp signal. * Present address: Howard Hughes Medical Institute, Columbia University, 701 West 168 Street, New York, NY 10032, USA 08 07 1992 © 1992 by Company of Biologists 1992 transcriptional induction homeotic gene autoregulation growth factor...